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dc.contributor.authorWang, Z.
dc.contributor.authorZhang, T.
dc.contributor.authorXie, S.
dc.contributor.authorLiu, X.
dc.contributor.authorLi, H.
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorChi, L.
dc.date2018
dc.date.accessioned2022-06-27T15:52:27Z
dc.date.available2022-06-27T15:52:27Z
dc.date.issued2018
dc.identifier.citationSequencing the Oligosaccharide Pool in Dalteparin Sodium with Offline Strong Anion Exchange (SAX)-HPLC and ESI-MS/MS, Z. Wang, T. Zhang, S. Xie, X. Liu, H. Li, R. J. Linhardt, L. Chi, Carbohydrate Polymers, 183, 81-90, 2018.
dc.identifier.urihttps://doi.org/10.1016/j.carbpol.2017.11.039
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5604
dc.descriptionCarbohydrate Polymers, 183, 81-90
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractLow molecular weight heparins (LMWHs) are widely used anticoagulant drugs. The composition and sequence of LMWH oligosaccharides determine their safety and efficacy. The short oligosaccharide pool in LMWHs undergoes more depolymerization reactions than the longer chains and is the most sensitive indicator of the manufacturing process. Electrospray ionization tandem mass spectrometry (ESI-MS/MS) has been demonstrated as a powerful tool to sequence synthetic heparin oligosaccharide but never been applied to analyze complicated mixture like LMWHs. We established an offline strong anion exchange (SAX)-high performance liquid chromatography (HPLC) and ESI-MS/MS approach to sequence the short oligosaccharides of dalteparin sodium. With the help of in-house developed MS/MS interpretation software, the sequences of 18 representative species ranging from tetrasaccharide to octasaccharide were obtained. Interestingly, we found a novel 2,3-disulfated hexauronic acid structure and reconfirmed it by complementary heparinase digestion and LC-MS/MS analysis. This approach provides straightforward and in-depth insight to the structure of LMWHs and the reaction mechanism of heparin depolymerization.
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/j.carbpol.2017.11.039
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleSequencing the Oligosaccharide Pool in Dalteparin Sodium with Offline Strong Anion Exchange (SAX)-HPLC and ESI-MS/MS
dc.typeArticle
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dcterms.isVersionOfhttps://doi.org/10.1016/j.carbpol.2017.11.039
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)


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