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dc.contributor.authorWang, Haisheng
dc.contributor.authorHe, Wenqin
dc.contributor.authorJiang, Peixia
dc.contributor.authorYu, Yanlei
dc.contributor.authorLin, Lei
dc.contributor.authorSun, Xiaojun
dc.contributor.authorKoffas, Mattheos
dc.contributor.authorZhang, Fuming
dc.contributor.authorLinhardt, Robert J.
dc.date2017
dc.date.accessioned2022-06-27T15:52:27Z
dc.date.available2022-06-27T15:52:27Z
dc.date.issued2017-10-01
dc.identifier.citationConstruction and functional characterization of truncated versions of recombinant keratanase II from Bacillus circulans, H. Wang, W. He, P. Jiang, Y. Yu, L. Lin, X. Sun, M. Koffas, F. Zhang, Robert J. Linhardt, Glycoconjugate Journal, 34, 643–649, 2017.
dc.identifier.issn15734986
dc.identifier.issn2820080
dc.identifier.urihttps://doi.org/10.1007/s10719-017-9786-3
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5614
dc.descriptionGlycoconjugate Journal, 34, 643–649
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractThere is a need for degradative enzymes in the study of glycosaminoglycans. Many of these enzymes are currently available either in their natural or recombinant forms. Unfortunately, progress in structure-activity studies of keratan sulfate (KS) have been impeded by the lack of a commercially available endo-β-N-acetylglucosaminidase, keratantase II. The current study uses a recently published sequence of a highly thermostable keratanase II identified in Bacillus circulans to clone and express a series of truncation mutants in Escherichia coli BL21. The resulting truncated forms of keratanase II exhibit activity and excellent storage and thermal stability making these useful tools for glycobiology research.
dc.description.sponsorshipFoundation for the National Institutes of Health
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1007/s10719-017-9786-3
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofGlycoconjugate Journal
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleConstruction and functional characterization of truncated versions of recombinant keratanase II from Bacillus circulans
dc.typeArticle
dcterms.accessRightshttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1007/s10719-017-9786-3
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1007/s10719-017-9786-3
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages643-649
rpi.description.volume34


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