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dc.contributor.authorXiu, Yu
dc.contributor.authorJang, Sungho
dc.contributor.authorJones, J. Andrew
dc.contributor.authorZill, Nicholas A.
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorYuan, Qipeng
dc.contributor.authorJung, Gyoo Yeol
dc.contributor.authorKoffas, Mattheos A.G.
dc.date2017
dc.date.accessioned2022-06-27T16:01:10Z
dc.date.available2022-06-27T16:01:10Z
dc.date.issued2017-10-01
dc.identifier.citationNaringenin-Responsive Riboswitch-Based Fluorescent Biosensor Module for Escherichia coli Co-Cultures, Y. Xiu, S. Jang, J. A. Jones, N. A. Zill, R. J. Linhardt, Q. Yuan, G. Yeol Jung, M. A.G. Koffas, Biotechnology and Bioengineering, 114, 2235-2244, 2017.
dc.identifier.issn10970290
dc.identifier.issn63592
dc.identifier.urihttps://doi.org/10.1002/bit.26340
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5615
dc.descriptionBiotechnology and Bioengineering, 114, 2235-2244
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractThe ability to design and construct combinatorial synthetic metabolic pathways has far exceeded our capacity for efficient screening and selection of the resulting microbial strains. The need for high-throughput rapid screening techniques is of upmost importance for the future of synthetic biology and metabolic engineering. Here we describe the development of an RNA riboswitch-based biosensor module with dual fluorescent reporters, and demonstrate a high-throughput flow cytometry-based screening method for identification of naringenin over producing Escherichia coli strains in co-culture. Our efforts helped identify a number of key operating parameters that affect biosensor performance, including the selection of promoter and linker elements within the sensor-actuator domain, and the effect of host strain, fermentation time, and growth medium on sensor dynamic range. The resulting biosensor demonstrates a high correlation between specific fluorescence of the biosensor strain and naringenin titer produced by the second member of the synthetic co-culture system. This technique represents a novel application for synthetic microbial co-cultures and can be expanded from naringenin to any metabolite if a suitable riboswitch is identified. The co-culture technique presented here can be applied to a variety of target metabolites in combination with the SELEX approach for aptamer design. Due to the compartmentalization of the two genetic constructs responsible for production and detection into separate cells and application as independent modules of a synthetic microbial co-culture we have subsequently reduced the need for re-optimization of the producer module when the biosensor is replaced or removed. Biotechnol. Bioeng. 2017;114: 2235-2244.
dc.description.sponsorshipNational Natural Science Foundation of China
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1002/bit.26340
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofBiotechnology and Bioengineering
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleNaringenin-Responsive Riboswitch-Based Fluorescent Biosensor Module for Escherichia coli Co-Cultures
dc.typeArticle
dcterms.accessRightshttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1002/bit.26340
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dcterms.isVersionOfhttps://doi.org/10.1002/bit.26340
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages2235-2244
rpi.description.volume114


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