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dc.contributor.authorRong, Yinxiu
dc.contributor.authorXu, Naiyu
dc.contributor.authorXie, Bingying
dc.contributor.authorHao, Jie
dc.contributor.authorYi, Lin
dc.contributor.authorCheng, Ruomei
dc.contributor.authorLi, Duxin
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorZhang, Zhenqing
dc.date2017
dc.date.accessioned2022-06-27T16:01:10Z
dc.date.available2022-06-27T16:01:10Z
dc.date.issued2017-12-01
dc.identifier.citationSequencing analysis of β-glucan from highland barley with high performance anion exchange chromatography coupled to quadrupole time–of–flight mass spectrometry, Y. Rong, N. Xu, R. Cheng, B. Xie, J. Hao, L. Yi, D. Li, R. J. Linhardt, Z. Zhang, Food Hydrocolloids, 73, 235-242, 2017.
dc.identifier.issn0268005X
dc.identifier.urihttps://doi.org/10.1016/j.foodhyd.2017.07.006
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5621
dc.descriptionFood Hydrocolloids, 73, 235-242
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractHighland barley is one of the hull-less barleys that contains high amounts of β-glucan. A detailed sequence analysis is needed to support the application of the β-glucan from highland barley. The β-conformation and 1 → 3/1 → 4 linkages were confirmed using nuclear magnetic resonance (NMR) spectroscopy. Monosaccharide analysis was accomplished with high-performance anion exchange chromatography (HPAEC) coupled to pulsed amperometric detection (PAD) and showed that glucose (G) was the dominant sugar component of this β-glucan. HPAEC-PAD was applied with on-line electrospray ion - to quadrupole time – of – flight mass spectrometry (Q/TOF-MS) to sequence oligosaccharides enzymatically derived from this β-glucan. More than 20 oligosaccharides were observed in the partially digested β-glucan mixture and their degree of polymerization (dp), confirmed by MS analysis, ranged from dp 2 to dp 20. The sequences of oligosaccharide dp2 – dp7 in the digested product mixture were unambiguously determined with MS/MS. Based on the sequence analysis of these oligosaccharides, G1→3G1→4G and G1→3G1→4G1→4G were determined to be the major repeating blocks of this β-glucan, and they were interspersed with homogeneous 1 → 4 linked domains with different lengths. The ratio of 1 → 3 and 1 → 4 linkages in the β-glucan was approximately 1:3.
dc.description.sponsorshipNational Natural Science Foundation of China
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/j.foodhyd.2017.07.006
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofFood Hydrocolloids
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleSequencing analysis of β-glucan from highland barley with high performance anion exchange chromatography coupled to quadrupole time–of–flight mass spectrometry
dc.typeArticle
dcterms.accessRightshttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/j.foodhyd.2017.07.006
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dcterms.isVersionOfhttps://doi.org/10.1016/j.foodhyd.2017.07.006
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages235-242
rpi.description.volume73


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