dc.contributor.author | Fulcher, Yan G. | |
dc.contributor.author | Prior, Stephen H. | |
dc.contributor.author | Masuko, Sayaka | |
dc.contributor.author | Li, Lingyun | |
dc.contributor.author | Pu, Dennis | |
dc.contributor.author | Zhang, Fuming | |
dc.contributor.author | Linhardt, Robert J. | |
dc.contributor.author | Van Doren, Steven R. | |
dc.date | 2017 | |
dc.date.accessioned | 2022-06-27T16:01:10Z | |
dc.date.available | 2022-06-27T16:01:10Z | |
dc.date.issued | 2017-07-05 | |
dc.identifier.citation | Glycan Activation of a Sheddase: Electrostatic Recognition between Heparin and proMMP-7, Y. G. Fulcher, S. H. Prior, S. Masuko, L. Li, D. Pu, F. Zhang, R. J. Linhardt, S. R. Van Doren, Structure, 25, 1100–1110, 2017. | |
dc.identifier.issn | 18784186 | |
dc.identifier.issn | 9692126 | |
dc.identifier.uri | https://doi.org/10.1016/j.str.2017.05.019 | |
dc.identifier.uri | https://hdl.handle.net/20.500.13015/5622 | |
dc.description | Structure, 25, 1100–1110 | |
dc.description | Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform. | |
dc.description.abstract | Heparan sulfate proteoglycans activate the matrix metalloproteinase-7 zymogen (proMMP-7) and recruit it in order to shed proteins from cell surfaces. This occurs in uterine and mammary epithelia, bacterial killing, lung healing, and tumor cell signaling. Basic tracks on proMMP-7 recognize polyanionic heparin, according to nuclear magnetic resonance and mutations disruptive of maturation. Contacts and proximity measurements guided docking of a heparin octasaccharide to proMMP-7. The reducing end fits into a basic pocket in the pro-domain while the chain continues toward the catalytic domain. Another oligosaccharide traverses a basic swath remote on the catalytic domain and inserts its reducing end into a slot formed with the basic C terminus. This latter association appears to support allosteric acceleration of proteolysis. The modes of binding account for extended, heterogeneous assemblies of proMMP-7 with heparinoids during maturation and for bridging to pro-α-defensins and proteoglycans. These associations support proteolytic release of activities at epithelial cell surfaces. | |
dc.description.sponsorship | National Institutes of Health | |
dc.description.uri | https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/j.str.2017.05.019 | |
dc.language | en_US | |
dc.language.iso | ENG | |
dc.relation.ispartof | The Linhardt Research Labs Online Collection | |
dc.relation.ispartof | Rensselaer Polytechnic Institute, Troy, NY | |
dc.relation.ispartof | Structure | |
dc.relation.uri | https://harc.rpi.edu/ | |
dc.subject | Biology | |
dc.subject | Chemistry and chemical biology | |
dc.subject | Chemical and biological engineering | |
dc.subject | Biomedical engineering | |
dc.title | Glycan Activation of a Sheddase: Electrostatic Recognition between Heparin and proMMP-7 | |
dc.type | Article | |
dcterms.accessRights | https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/j.str.2017.05.019 | |
dcterms.isPartOf | Journal | |
dcterms.isVersionOf | https://doi.org/10.1016/j.str.2017.05.019 | |
dc.rights.holder | In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/ | |
dc.creator.identifier | https://orcid.org/0000-0003-2219-5833 | |
dc.relation.department | The Linhardt Research Labs. | |
dc.relation.department | The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS) | |
rpi.description.pages | 1100-1110.e5 | |
rpi.description.volume | 25 | |