dc.contributor.author | Wei, Chao Yang | |
dc.contributor.author | Liao, Ning Bo | |
dc.contributor.author | Zhang, Yu | |
dc.contributor.author | Ye, Xing Qian | |
dc.contributor.author | Li, Shan | |
dc.contributor.author | Hu, Ya Qin | |
dc.contributor.author | Liu, Dong Hong | |
dc.contributor.author | Linhardt, Robert J. | |
dc.contributor.author | Wang, Xin | |
dc.contributor.author | Chen, Shi Guo | |
dc.date | 2017 | |
dc.date.accessioned | 2022-06-27T16:01:10Z | |
dc.date.available | 2022-06-27T16:01:10Z | |
dc.date.issued | 2017-09-01 | |
dc.identifier.citation | In vitro fermentation behaviors of fucosylated chondroitin sulfate from Pearsonothuria graeffei by human gut microflora, C. Wei, Y. Zhang, N. Liao, S. Li, R. Linhardt, X. Wang, X. Ye, S. Chen, International Journal of Biological Macromolecules, 102, 1195–1201, 2017. | |
dc.identifier.issn | 18790003 | |
dc.identifier.issn | 1418130 | |
dc.identifier.uri | https://doi.org/10.1016/j.ijbiomac.2017.04.036 | |
dc.identifier.uri | https://hdl.handle.net/20.500.13015/5627 | |
dc.description | International Journal of Biological Macromolecules, 102, 1195–1201 | |
dc.description | Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform. | |
dc.description.abstract | A fucosylated chondroitin sulfate (FCS-pg) with highly repeated structure from Pearsonothuria graeffei was subjected to a in vitro fermentation model to investigate its fermentability and effects on human gut microflora. High performance liquid chromatography (HPLC) measurement found FCS-pg can be fermented to short chain fatty acids (SCFAs) by gut microflora from partial human fecal samples. 16S rRNA gene-based polymerase chain reaction-based denaturing gradient gel electrophoresis (PCR-DGGE) profiling and real-time quantitative PCR analysis showed that FCS-pg mainly increased the proportions of Clostridium cluster XI, Bacteriodes prevotella group, Bifidobacterium genus, Clostridium cluster I and Clostridium cluster XIVab, whereas the numbers of the Enterobacteriaceae and Lactobacillus decreased. These results indicated that FCS-pg was mainly fermented by Bacteroides, Bifidobacterium and Clostridium. It increased the content of probiotics bacteria in achieving health-enhancing effect, was slightly different than most sulfated polysaccharides from marine animals. The current study provides useful new information on the mechanism of absorption and functional activity on FCS-pg within the gastrointestinal tract of the human body. | |
dc.description.sponsorship | National Natural Science Foundation of China | |
dc.description.uri | https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/j.ijbiomac.2017.04.036 | |
dc.language | en_US | |
dc.language.iso | ENG | |
dc.relation.ispartof | The Linhardt Research Labs Online Collection | |
dc.relation.ispartof | Rensselaer Polytechnic Institute, Troy, NY | |
dc.relation.ispartof | International Journal of Biological Macromolecules | |
dc.relation.uri | https://harc.rpi.edu/ | |
dc.subject | Biology | |
dc.subject | Chemistry and chemical biology | |
dc.subject | Chemical and biological engineering | |
dc.subject | Biomedical engineering | |
dc.title | In vitro fermentation behaviors of fucosylated chondroitin sulfate from Pearsonothuria graeffei by human gut microflora | |
dc.type | Article | |
dcterms.accessRights | https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/j.ijbiomac.2017.04.036 | |
dcterms.isPartOf | Journal | |
dcterms.isVersionOf | https://doi.org/10.1016/j.ijbiomac.2017.04.036 | |
dc.rights.holder | In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/ | |
dc.creator.identifier | https://orcid.org/0000-0003-2219-5833 | |
dc.relation.department | The Linhardt Research Labs. | |
dc.relation.department | The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS) | |
rpi.description.pages | 1195-1201 | |
rpi.description.volume | 102 | |