Author
Englaender, Jacob A.; Zhu, Yuanyuan; Shirke, Abhijit N.; Lin, Lei; Liu, Xinyue; Zhang, Fuming; Gross, Richard A.; Koffas, Mattheos A.G.; Linhardt, Robert J.
Other Contributors
Date Issued
2017-04-01
Subject
Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
Degree
Terms of Use
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Full Citation
Expression and secretion of glycosylated heparin biosynthetic enzymes using Komagataella pastoris, J. A. Englaender, Y. Zhu, A.N. Shirke, L. Lin, X. Liu, F. Zhang, R.A. Gross, M.A.G. Koffas, R. J. Linhardt, Applied Microbiology and Biotechnology, 101, 2843–2851, 2017
Abstract
Heparin, an anticoagulant drug, is biosynthesized in selected animal cells. The heparin biosynthetic enzymes mainly consist of sulfotransferases and all are integral transmembrane glycoproteins. These enzymes are generally produced in engineered Escherichia coli as without their transmembrane domains as non-glycosylated fusion proteins. In this study, we used the yeast, Komagataella pastoris, to prepare four sulfotransferases involved in heparin biosynthesis as glycoproteins. While the yields of these yeast-expressed enzymes were considerably lower than E. coli-expressed enzymes, these enzymes were secreted into the fermentation media simplifying their purification and were endotoxin free. The activities of these sulfotransferases, expressed as glycoproteins in yeast, were compared to the bacterially expressed proteins. The yeast-expressed sulfotransferase glycoproteins showed improved kinetic properties than the bacterially expressed proteins.;
Description
Applied Microbiology and Biotechnology, 101, 2843–2851; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Department
The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
Relationships
The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Applied Microbiology and Biotechnology; https://harc.rpi.edu/;
Access
https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1007/s00253-016-8047-x;