Liquid to liquid extraction and liquid chromatography-tandem mass spectrometry determination of hainanmycin in feed

Authors
Wang, Ze Ping
Shen, Jian Zhong
Linhardt, Robert J.
Jiang, Hui
Cheng, Lin Li
ORCID
https://orcid.org/0000-0003-2219-5833
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Other Contributors
Issue Date
2017-03-01
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Degree
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Full Citation
Liquid to liquid extraction and liquid chromatography-tandem mass spectrometry determination of hainanmycin in feed, Z. P. Wang, J. Z. Shen, R. Linhardt, H. Jiang, L. L. Cheng, Journal of Chromatography B, 1046, 98–101, 2017.
Abstract
Hainanmycin is a new veterinary polyether antibiotic and has few sensitive analytical method in present days. In this study, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) relying on multiple reaction monitoring (MRM) detection was developed for analysis of hainanmycin in animal feed. Feed samples were extracted with ethyl acetate and purified by two steps of liquid-liquid extraction (LLE) to get rid of water solvable matrix and lipids one by one. The final simple was analyzed by LC-MS/MS. The LC mobile phase was composed of 0.1% aqueous formic acid and 0.1% formic acidified acetonitrile by gradient elution. Average recoveries ranged from 74.22% to 87.85%, as determined by spiking with 2.0 (LOQ) ∼2500μgkg-1 of hainanmycin. The inter-day and intra-day coefficient of variation was 9.21% to 11.77% and 7.67% to 13.49%, respectively. The limit of detection (LOD) and the limit of quantitation (LOQ) were 0.36μgkg-1 and 2.0μgkg-1, respectively.
Description
Journal of Chromatography B, 1046, 98–101
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Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
https://harc.rpi.edu/
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