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dc.contributor.authorYu, Yanlei
dc.contributor.authorChen, Yin
dc.contributor.authorMikael, Paiyz
dc.contributor.authorZhang, Fuming
dc.contributor.authorStalcup, Apryll M.
dc.contributor.authorGerman, Rebecca
dc.contributor.authorGould, Francois
dc.contributor.authorOhlemacher, Jocelyn
dc.contributor.authorZhang, Hong
dc.contributor.authorLinhardt, Robert J.
dc.date2017
dc.date.accessioned2022-06-27T16:01:44Z
dc.date.available2022-06-27T16:01:44Z
dc.date.issued2017-01-01
dc.identifier.citationSurprising absence of heparin in the intestinal mucosa of baby pigs, Y. Yu, Y. Chen, P. Mikael, F. Zhang, A. M. Stalcup, R. German, F. Gould, J. Ohlemacher, H. Zhang, R. J. Linhardt, Glycobiology, 27, 57–63, 2017
dc.identifier.issn14602423
dc.identifier.issn9596658
dc.identifier.urihttps://doi.org/10.1093/glycob/cww104
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5644
dc.descriptionGlycobiology, 27, 57–63
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractHeparin, a member of a family of molecules called glycosaminoglycans, is biosynthesized in mucosal mast cells. This important anticoagulant polysaccharide is primarily produced by extraction of the mast cell-rich intestinal mucosa of hogs. There is concern about our continued ability to supply sufficient heparin to support the worldwide growth of advanced medical procedures from the static population of adult hogs used as food animals. While the intestinal mucosa of adult pigs is rich in anticoagulant heparin (containing a few hundred milligrams per animal), little is known about how the content of heparin changes with animal age. Using sophisticated mass spectral analysis we discovered that heparin was largely absent from the intestinal mucosa of piglets. Moreover, while the related, nonanticoagulant heparan sulfate glycosaminoglycan was present in significant amounts we found little chondroitin sulfate E also associated with mast cells. Histological evaluation of piglet intestinal mucosa showed a very low mast cell content. Respiratory mast cells have been reported in baby pigs suggesting that there was something unique about the piglets used in the current study. These piglets were raised in the relatively clean environment of a university animal facility and treated with antibiotics over their lifetime resulting in a depleted microbiome that greatly reduced the number of mast cells and heparin content of the intestinal mucosal in these animals. Thus, from the current study it remains unclear whether the lack of intestinal mast cell-derived heparin results from the young age of these animals or their exposure to their depleted microbiome.
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofGlycobiology
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleSurprising absence of heparin in the intestinal mucosa of baby pigs
dc.typeArticle
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1093/glycob/cww104
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages57-63
rpi.description.volume27


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