Keratan sulfate glycosaminoglycan from chicken egg white
Authors
Fu, Li
Sun, Xiaojun
He, Wenqin
Cai, Chao
Onishi, Akihiro
Zhang, Fuming
Linhardt, Robert J.
Liu, Zhangguo
ORCID
https://orcid.org/0000-0003-2219-5833
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Other Contributors
Issue Date
2016-07-01
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Degree
Terms of Use
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Full Citation
Keratan sulfate glycosaminoglycan from chicken egg white, L. Fu, X. Sun, W. He, C. Cai, A. Onishi, F. Zhang, R. J. Linhardt, Z. Liu, Glycobiology, 26, 693–700, 2016.
Abstract
Keratan sulfate (KS) was isolated from chicken egg white in amounts corresponding to ∼0.06 wt% (dry weight). This KS had a weight-average molecular weight of ∼36–41 kDa with a polydispersity of ∼1.3. The primary repeating unit present in chicken egg white KS was →4) β-N-acetyl-6-O-sulfo-D-glucosamine (1 → 3) β-D-galactose (1→ with some 6-O-sulfo galactose residues present. This KS was somewhat resistant to depolymerization using keratanase 1 but could be depolymerized efficiently through the use of reactive oxygen species generated using copper (II) and hydrogen peroxide. Of particular interest was the presence of substantial amounts of 2,8- and 2,9-linked N-acetylneuraminic acid residues in the form of oligosialic acid terminating the non-reducing ends of the KS chains. Most of the KS appears to be N-linked to a protein core as evidenced by its sensitivity to PNGase F.
Description
Glycobiology, 26, 693–700
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Glycobiology
https://harc.rpi.edu/
Rensselaer Polytechnic Institute, Troy, NY
Glycobiology
https://harc.rpi.edu/