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dc.contributor.authorGe, Dan
dc.contributor.authorHigashi, Kyohei
dc.contributor.authorIto, Daichi
dc.contributor.authorNagano, Kenichi
dc.contributor.authorIshikawa, Ryota
dc.contributor.authorTerui, Yusuke
dc.contributor.authorHigashi, Kenjirou
dc.contributor.authorMoribe, Kunikazu
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorToida, Toshihiko
dc.identifier.citationPoly-ion Complex of Chondroitin Sulfate and Spermine and its Effect on Oral Chondroitin Sulfate Bioavailability, D. Ge, K. Higashi, D. Ito, Y. Terui K. Nagano, R. Ishikawa, K. Higashi, K. Moribe, R. J. Linhardt, T. Toida, Bulletins of the Japanese Pharmaceutical Society, 64, 390–398, 2016.
dc.descriptionBulletins of the Japanese Pharmaceutical Society, 64, 390–398
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractChondroitin sulfate (CS) has been accepted as an ingredient in health foods for the treatment of symptoms related to arthritis and cartilage repair. However, CS is poorly absorbed through the gastrointestinal tract because of its high negative electric charges and molecular weight (MW). In this study, poly-ion complex (PIC) formation was found in aqueous solutions through electrostatic interaction between CS and polyamines-organic molecules having two or more primary amino groups ubiquitously distributed in natural products at high concentrations. Characteristic properties of various PICs generated by mixing CS and natural polyamines, including unusual polyamines, were studied based on the turbidity for PIC formation, the dynamic light scattering for the size of PIC particles, and ζ-potential measurements for the surface charges of PIC particles. The efficiency of PIC formation between CS and spermine increased in a CS MW-dependent manner, with 15 kDa CS being critical for the formation of PIC (particle size: 3.41 µm) having nearly neutral surface charge (ζ-potential: -0.80 mV). Comparatively, mixing tetrakis(3-aminopropyl)ammonium and 15 kDa of CS afforded significant levels of PIC (particle size: 0.42±0.16 µm) despite a strongly negative surface charge (-34.67±1.15 mV). Interestingly, the oral absorption efficiency of CS was greatly improved only when PIC possessing neutral surface charges was administered to mice. High formation efficiency and electrically neutral surface charge of PIC particles are important factors for oral CS bioavailability.
dc.description.sponsorshipJapan Society for the Promotion of Science
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofChemical and Pharmaceutical Bulletin
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titlePoly-ion Complex of Chondroitin Sulfate and Spermine and its Effect on Oral Chondroitin Sulfate Bioavailability
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)

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