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dc.contributor.authorMora-Pale, M.
dc.contributor.authorBhan, N.
dc.contributor.authorMasuko, S.
dc.contributor.authorJames, P.
dc.contributor.authorWood, J.
dc.contributor.authorMcCallum, S.
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorDordick, J.S.
dc.contributor.authorKoffas, M.A.G.
dc.date2015
dc.date.accessioned2022-06-27T16:04:32Z
dc.date.available2022-06-27T16:04:32Z
dc.date.issued2015
dc.identifier.citationAntimicrobial mechanism of resveratrol-trans-dihydrodimer produced from peroxidase-mediated oligomerization of resveratrol, M. Mora-Pale, N. Bhan, S. Masuko, P. James, J. Wood, S. McCallum, R. J. Linhardt, J. S. Dordick, M. A.G. Koffas, Biotechnology and Bioengineering, 112, 2417-2428, 2015.
dc.identifier.urihttps://doi.org/10.1002/bit.25686
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5669
dc.descriptionBiotechnology and Bioengineering, 112, 2417-2428
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractPlant polyphenols are known to have varying antimicrobial potencies, including direct antibacterial activity, synergism with antibiotics and suppression of bacterial virulence. We performed the in vitro oligomerization of resveratrol catalyzed by soybean peroxidase, and the two isomers (resveratrol-trans-dihydrodimer and pallidol) produced were tested for antimicrobial activity. The resveratrol-trans-dihydrodimer displayed antimicrobial activity against the Gram-positive bacteria Bacillus cereus, Listeria monocytogenes, and Staphylococcus aureus (minimum inhibitory concentration (MIC) = 15.0, 125, and 62.0 μM, respectively) and against Gram-negative Escherichia coli (MIC = 123 μM, upon addition of the efflux pump inhibitor Phe-Arg-β-naphthylamide). In contrast, pallidol had no observable antimicrobial activity against all tested strains. Transcriptomic analysis implied downregulation of ABC transporters, genes involved in cell division and DNA binding proteins. Flow cytometric analysis of treated cells revealed a rapid collapse in membrane potential and a substantial decrease in total DNA content. The active dimer showed >90% inhibition of DNA gyrase activity, in vitro, by blocking the ATP binding site of the enzyme. We thus propose that the resveratrol-trans-dihydrodimer acts to: (1) disrupt membrane potential; and (2) inhibit DNA synthesis. In summary, we introduce the mechanisms of action and the initial evaluation of an active bactericide, and a platform for the development of polyphenolic antimicrobials.
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1002/bit.25686
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleAntimicrobial mechanism of resveratrol-trans-dihydrodimer produced from peroxidase-mediated oligomerization of resveratrol
dc.typeArticle
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dcterms.isVersionOfhttps://doi.org/10.1002/bit.25686
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dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)


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