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dc.contributor.authorHigashi, Kyohei
dc.contributor.authorOkamoto, Yusuke
dc.contributor.authorMukuno, Ann
dc.contributor.authorWakai, Jun
dc.contributor.authorHosoyama, Saori
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorToida, Toshihiko
dc.identifier.citationFunctional chondroitin sulfate from Enteroctopus dofleini containing a 3-O-sulfo glucuronic acid residue, K. Higashi, Y. Okamoto, A. Mukuno, J. Wakai, S. Hosoyama, R.J. Linhardt, T. Toida, Carbohydrate Polymers, 134, 557–565, 2015.
dc.descriptionCarbohydrate Polymers, 134, 557–565
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractThere are several reports that chondroitin sulfate containing K-type units [GlcA (3S)-GalNAc (4S)] exhibiting similar levels of neurite outgrowth promoting activities as CS having high amounts of B-, D- and E-type disulfated disaccharides. Although CS containing K-type units possess important biological activities, there are only few sources, such as king crab cartilage, squid cartilage or sea cucumber. In this study, CS containing 13.9% of K-type units was found in octopus (Enteroctopus dofleini) cartilage using different substrate specificities of chondroitinases. The 2D NMR spectra showed cross-peaks assigned to protons on sugar ring of GlcA (3S), demonstrating the presence of K-type units in octopus CS. Furthermore, proportion of fucosylated disaccharide units in octopus CS was very low. Octopus CS showed high affinity for growth factors and stimulated neurite outgrowth of hippocampal neurons, similar to the activity of squid CS-E. These results strongly suggest that octopus cartilage is a rich source of CS-K and has important biological activities.
dc.description.sponsorshipJapan Society for the Promotion of Science
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofCarbohydrate Polymers
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleFunctional chondroitin sulfate from Enteroctopus dofleini containing a 3-O-sulfo glucuronic acid residue
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)

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