Show simple item record

dc.contributor.authorZhang, J.
dc.contributor.authorSuflita, M.
dc.contributor.authorLi, G.
dc.contributor.authorZhong, W.
dc.contributor.authorLi, L.
dc.contributor.authorZhang, F.
dc.contributor.authorDordick, J.S.
dc.contributor.authorLinhardt, Robert J.
dc.date2015
dc.date.accessioned2022-06-27T16:05:22Z
dc.date.available2022-06-27T16:05:22Z
dc.date.issued2015
dc.identifier.citationHigh cell density cultivation of recombinant Escherichia coli strains expressing 2-O-sulfotransferase and C5-epimerase for the production of bioengineering heparin, J. Zhang, M. Suflita, G. Li, W. Zhong, L. Li, F. Zhang, J. S. Dordick, R. J. Linhardt, Applied Biochemistry and Biotechnology, 175, 2986–2995, 2015.
dc.identifier.urihttps://doi.org/10.1007/s12010-014-1466-1
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5685
dc.descriptionApplied Biochemistry and Biotechnology, 175, 2986–2995
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractBioengineered heparin is being investigated as a potential substitute for the animal-sourced anticoagulant drug. One step in the current process to prepare bioengineered heparin involves the conversion of N-sulfo heparosan, rich in → 4)GlcNS(1 → 4) GlcA(1 → sequences (where S is sulfo, GlcN is α-D-glucosamine, and GlcA is β-D-glucuronic acid), to a critical intermediate, rich in → 4)GlcNS(1 → 4) IdoA2S(1 → sequences (where S is sulfo and IdoA is α-L-iduronic acid), using 2-O-sulfotransferase (2-OST) and C5 epimerase (C5-epi). Until now, these heparan sulfate biosynthetic enzymes have been expressed in Escherichia coli grown in shake flask culture as fusion proteins. The current study is focused on the high cell density fed-batch cultivation of recombinant E. coli strains expressing both enzymes. We report the high productivity expression of active 2-OST and C5-epi enzymes of 6.0 and 2.2 mg/g dry cell weight, respectively.
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1007/s12010-014-1466-1
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleHigh cell density cultivation of recombinant Escherichia coli strains expressing 2-O-sulfotransferase and C5-epimerase for the production of bioengineering heparin
dc.typeArticle
dcterms.accessRightshttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1007/s12010-014-1466-1
dcterms.isVersionOfhttps://doi.org/10.1007/s12010-014-1466-1
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record