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dc.contributor.authorDatta, Payel
dc.contributor.authorYang, Bo
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorSharfstein, Susan T.
dc.date2015
dc.date.accessioned2022-06-27T16:05:22Z
dc.date.available2022-06-27T16:05:22Z
dc.date.issued2015-03-18
dc.identifier.citationModulation of heparan sulfate biosynthesis by sodium butyrate in recombinant CHO cells, P. Datta, B. Yang, R. J. Linhardt, S.T. Sharfstein, Cytotechnology, 67, 223–235, 2015.
dc.identifier.issn15730778
dc.identifier.issn9209069
dc.identifier.urihttps://doi.org/10.1007/s10616-013-9677-9
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5686
dc.descriptionCytotechnology, 67, 223–235
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractSodium butyrate, a histone deacetylase inhibitor, has been used to improve transgene expression in Chinese hamster ovary (CHO) cells. The current study explores the impact of butyrate treatment on heparan sulfate (HS) biosynthesis and structural composition in a recombinant CHO-S cell line expressing enzymes in the heparin (HP)/(HS) biosynthetic pathway (Dual-10 stably expressing NDST2 and HS3st1). Flow cytometric analysis showed that antithrombin binding was increased in Dual-10 cells and basic fibroblast growth factor binding was decreased in response to sodium butyrate treatment. The results were in agreement with the AMAC-LCMS (2-aminoacridine-tagged HS/HP analysis by liquid chromatography mass spectrometry) data that showed that there was an increase in heparan sulfate tri-sulfated disaccharides and a decrease in N-sulfated disaccharides in the butyrate-treated cells. However, we could not detect any changes in the chondroitin sulfate pathway in Dual-10 cells treated with butyrate. The current study is the first to report the effect of butyrate on glycosaminoglycan profiles.
dc.description.sponsorshipNational Institutes of Health
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1007/s10616-013-9677-9
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofCytotechnology
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleModulation of heparan sulfate biosynthesis by sodium butyrate in recombinant CHO cells
dc.typeArticle
dcterms.accessRightshttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1007/s10616-013-9677-9
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1007/s10616-013-9677-9
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages223-235
rpi.description.volume67


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