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dc.contributor.authorHe, Wenqin
dc.contributor.authorFu, Li
dc.contributor.authorLi, Guoyun
dc.contributor.authorAndrew Jones, J.
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorKoffas, Mattheos
dc.date2015
dc.date.accessioned2022-06-27T16:05:22Z
dc.date.available2022-06-27T16:05:22Z
dc.date.issued2015-01-01
dc.identifier.citationProduction of chondroitin in metabolically engineered E. coli, W. He, L. Fu, G. Li, J. A. Jones, R. J. Linhardt, M. Koffas, Metabolic Engineering, 27, 92-100, 2015.
dc.identifier.issn10967184
dc.identifier.issn10967176
dc.identifier.urihttps://doi.org/10.1016/j.ymben.2014.11.003
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5689
dc.descriptionMetabolic Engineering, 27, 92-100
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractChondroitin sulfates, widely used in the treatment of arthritis, are glycosaminoglycans extracted from food animal tissues. As part of our ongoing efforts to separate the food chain from the drug chain, we are examining the possibility of using metabolic engineering to produce chondroitin sulfate in Escherichia coli. Chondroitin is a valuable precursor in the synthesis of chondroitin sulfate. This study proposes a safer and more feasible approach to metabolically engineer chondroitin production by expressing genes from the pathogenic E. coli K4 strain, which natively produces a capsular polysaccharide that shares the similar structure with chondroitin, into the non-pathogenic E. coli BL21 Star™ (DE3) strain. The ePathBrick vectors, allowing for multiple gene addition and expression regulatory signal control, are used for metabolic balancing needed to obtain the maximum potential yield. The resulting engineered strain produced chondroitin, as demonstrated by (1)H NMR and disaccharide analysis, relying on chondrotinase treatment followed by liquid chromatography-mass spectrometry. The highest yield from shake flask experiment was 213mg/L and further increased to 2.4g/L in dissolved oxygen-stat fed batch bioreactor.
dc.description.sponsorshipNational Science Foundation
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/j.ymben.2014.11.003
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofMetabolic Engineering
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleProduction of chondroitin in metabolically engineered E. coli
dc.typeArticle
dcterms.accessRightshttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/j.ymben.2014.11.003
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1016/j.ymben.2014.11.003
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages92-100
rpi.description.volume27


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