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dc.contributor.authorGasimli, Leyla
dc.contributor.authorGlass, Charles A.
dc.contributor.authorDatta, Payel
dc.contributor.authorYang, Bo
dc.contributor.authorLi, Guoyun
dc.contributor.authorGemmill, Trent R.
dc.contributor.authorBaik, Jong Youn
dc.contributor.authorSharfstein, Susan T.
dc.contributor.authorEsko, Jeffrey D.
dc.contributor.authorLinhardt, Robert J.
dc.date2014
dc.date.accessioned2022-06-27T16:06:07Z
dc.date.available2022-06-27T16:06:07Z
dc.date.issued2014-03-01
dc.identifier.citationBioengineering murine mastocytoma cells to produce anticoagulant heparin, L. Gasimli, C. A. Glass, P. Datta, B. Yang, G. Li, T. R. Gemmill, J. Y. Baik, S. T. Sharfstein, J. D. Esko, R. J. Linhardt, Glycobiology, 24, 272-280, 2014
dc.identifier.issn14602423
dc.identifier.issn9596658
dc.identifier.urihttps://doi.org/10.1093/glycob/cwt108
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5697
dc.descriptionGlycobiology, 24, 272-280
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractHeparin (HP), an important anticoagulant polysaccharide, is produced in a complex biosynthetic pathway in connective tissue-type mast cells. Both the structure and size of HP are critical factors determining the anticoagulation activity. A murine mastocytoma (MST) cell line was used as a model system to gain insight into this pathway. As reported, MST cells produce a highly sulfated HP-like polysaccharide that lacks anticoagulant activity (Montgomery RI, Lidholt K, Flay NW, Liang J, Vertel B, Lindahl U, Esko JD. 1992. Stable heparin-producing cell lines derived from the Furth murine mastocytoma. Proc Natl Acad Sci USA 89:11327–11331). Here, we show that transfection of MST cells with a retroviral vector containing heparan sulfate 3-O-sulfotransferase-1 (Hs3st1) restores anticoagulant activity. The MST lines express N-acetylglucosamine N-deacetylase/N-sulfotransferase-1, uronosyl 2-O-sulfotransferase and glucosaminyl 6-O-sulfotransferase-1, which are sufficient to make the highly sulfated HP. Overexpression of Hs3st1 in MST-10H cells resulted in a change in the composition of heparan sulfate (HS)/HP and CS/dermatan sulfate (DS) glycosaminoglycans. The cell-associated HS/HP closely resembles HP with 3-O-sulfo group-containing glucosamine residues and shows anticoagulant activity. This study contributes toward a better understanding of the HP biosynthetic pathway with the goal of providing tools to better control the biosynthesis of HP chains with different structures and activities.
dc.description.sponsorshipNational Institutes of Health
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofGlycobiology
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleBioengineering murine mastocytoma cells to produce anticoagulant heparin
dc.typeArticle
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1093/glycob/cwt108
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages272-280
rpi.description.volume24


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