| dc.contributor.author | Huang, Haichan | |
| dc.contributor.author | Zhao, Yingying | |
| dc.contributor.author | Lv, Shencong | |
| dc.contributor.author | Zhong, Weihong | |
| dc.contributor.author | Zhang, Fuming | |
| dc.contributor.author | Linhardt, Robert J. | |
| dc.date | 2014 | |
| dc.date.accessioned | 2022-06-27T16:06:07Z | |
| dc.date.available | 2022-06-27T16:06:07Z | |
| dc.date.issued | 2014-02-15 | |
| dc.identifier.citation | Quantitation of heparosan with heparin lyase III and spectrophotometry, H. Huang, Y. Zhao, S. Lv, W. Zhong, F. Zhang，R. J. Linhardt, Analytical Biochemistry, 447, 46–48, 2014 | |
| dc.identifier.issn | 10960309 | |
| dc.identifier.issn | 32697 | |
| dc.identifier.uri | https://doi.org/10.1016/j.ab.2013.10.029 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.13015/5698 | |
| dc.description | Analytical Biochemistry, 447, 46–48 | |
| dc.description | Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform. | |
| dc.description.abstract | Heparosan is Escherichia coli K5 capsule polysaccharide, which is the key precursor for preparing bioengineered heparin. A rapid and effective quantitative method for detecting heparosan is important in the large-scale production of heparosan. Heparin lyase III (Hep III) effectively catalyzes the heparosan depolymerization, forming unsaturated disaccharides that are measurable using a spectrophotometer at 232 nm. We report a new method for the quantitative detection of heparosan with heparin lyase III and spectrophotometry that is safer and more specific than the traditional carbazole assay. In an optimized detection system, heparosan at a minimum concentration of 0.60 g/L in fermentation broth can be detected. | |
| dc.description.sponsorship | National Natural Science Foundation of China | |
| dc.description.uri | https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/j.ab.2013.10.029 | |
| dc.language | en_US | |
| dc.language.iso | ENG | |
| dc.relation.ispartof | The Linhardt Research Labs Online Collection | |
| dc.relation.ispartof | Rensselaer Polytechnic Institute, Troy, NY | |
| dc.relation.ispartof | Analytical Biochemistry | |
| dc.relation.uri | https://harc.rpi.edu/ | |
| dc.subject | Biology | |
| dc.subject | Chemistry and chemical biology | |
| dc.subject | Chemical and biological engineering | |
| dc.subject | Biomedical engineering | |
| dc.title | Quantitation of heparosan with heparin lyase III and spectrophotometry | |
| dc.type | Article | |
| dcterms.accessRights | https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/j.ab.2013.10.029 | |
| dcterms.isPartOf | Journal | |
| dcterms.isVersionOf | https://doi.org/10.1016/j.ab.2013.10.029 | |
| dc.rights.holder | In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/ | |
| dc.creator.identifier | https://orcid.org/0000-0003-2219-5833 | |
| dc.relation.department | The Linhardt Research Labs. | |
| dc.relation.department | The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS) | |
| rpi.description.pages | 46-48 | |
| rpi.description.volume | 447 | |
