Show simple item record

dc.contributor.authorHuang, Haichan
dc.contributor.authorZhao, Yingying
dc.contributor.authorLv, Shencong
dc.contributor.authorZhong, Weihong
dc.contributor.authorZhang, Fuming
dc.contributor.authorLinhardt, Robert J.
dc.date2014
dc.date.accessioned2022-06-27T16:06:07Z
dc.date.available2022-06-27T16:06:07Z
dc.date.issued2014-02-15
dc.identifier.citationQuantitation of heparosan with heparin lyase III and spectrophotometry, H. Huang, Y. Zhao, S. Lv, W. Zhong, F. Zhang,R. J. Linhardt, Analytical Biochemistry, 447, 46–48, 2014
dc.identifier.issn10960309
dc.identifier.issn32697
dc.identifier.urihttps://doi.org/10.1016/j.ab.2013.10.029
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5698
dc.descriptionAnalytical Biochemistry, 447, 46–48
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractHeparosan is Escherichia coli K5 capsule polysaccharide, which is the key precursor for preparing bioengineered heparin. A rapid and effective quantitative method for detecting heparosan is important in the large-scale production of heparosan. Heparin lyase III (Hep III) effectively catalyzes the heparosan depolymerization, forming unsaturated disaccharides that are measurable using a spectrophotometer at 232 nm. We report a new method for the quantitative detection of heparosan with heparin lyase III and spectrophotometry that is safer and more specific than the traditional carbazole assay. In an optimized detection system, heparosan at a minimum concentration of 0.60 g/L in fermentation broth can be detected.
dc.description.sponsorshipNational Natural Science Foundation of China
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/j.ab.2013.10.029
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofAnalytical Biochemistry
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleQuantitation of heparosan with heparin lyase III and spectrophotometry
dc.typeArticle
dcterms.accessRightshttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/j.ab.2013.10.029
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1016/j.ab.2013.10.029
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages46-48
rpi.description.volume447


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record