Expression of low endotoxin 3-O-sulfotransferase in Bacillus subtilis
Authors
Wang, Wenya
Englaender, Jacob A.
Xu, Peng
Mehta, Krunal K.
Suwan, Jiraporn
Dordick, Jonathan S.
Zhang, Fuming
Yuan, Qipeng
Linhardt, Robert J.
Koffas, Mattheos
ORCID
https://orcid.org/0000-0003-2219-5833
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Other Contributors
Issue Date
2013-10-01
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Degree
Terms of Use
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Full Citation
Expression of low endotoxin 3-O-sulfotransferase in Bacillus subtilis, W. Wang, P. Xu, J. Dordick, F. Zhang, Q. Yuan, M. Koffas, R.J. Linhardt, Applied Biochemistry and Biotechnology, 171, 954–962, 2013.
Abstract
A key enzyme for the biosynthesis and bioengineering of heparin, 3-O-sulfotransferase-1 (3-OST-1), was expressed and purified in Gram-positive Bacillus subtilis and Bacillus megaterium. Western blotting, protein sequence analysis, and enzyme activity measurement confirmed the expression. The enzymatic activity of 3-OST-1 expressed in Bacillus species were found to be similar to those found when expressed in Escherichia coli. The endotoxin level in 3-OST-1 from B. subtilis and B. megaterium were 10(4)-10(5)-fold lower than that of the E. coli-expressed 3-OST-1, which makes the Bacillus expression system of particular interest for the generation of pharmaceutical grade raw heparin from nonanimal sources.
Description
Applied Biochemistry and Biotechnology, 171, 954–962
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Applied Biochemistry and Biotechnology
https://harc.rpi.edu/
Rensselaer Polytechnic Institute, Troy, NY
Applied Biochemistry and Biotechnology
https://harc.rpi.edu/
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