Author
Wang, Wenya; Englaender, Jacob A.; Xu, Peng; Mehta, Krunal K.; Suwan, Jiraporn; Dordick, Jonathan S.; Zhang, Fuming; Yuan, Qipeng; Linhardt, Robert J.; Koffas, Mattheos
Other Contributors
Date Issued
2013-10-01
Subject
Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
Degree
Terms of Use
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Full Citation
Expression of low endotoxin 3-O-sulfotransferase in Bacillus subtilis, W. Wang, P. Xu, J. Dordick, F. Zhang, Q. Yuan, M. Koffas, R.J. Linhardt, Applied Biochemistry and Biotechnology, 171, 954–962, 2013.
Abstract
A key enzyme for the biosynthesis and bioengineering of heparin, 3-O-sulfotransferase-1 (3-OST-1), was expressed and purified in Gram-positive Bacillus subtilis and Bacillus megaterium. Western blotting, protein sequence analysis, and enzyme activity measurement confirmed the expression. The enzymatic activity of 3-OST-1 expressed in Bacillus species were found to be similar to those found when expressed in Escherichia coli. The endotoxin level in 3-OST-1 from B. subtilis and B. megaterium were 10(4)-10(5)-fold lower than that of the E. coli-expressed 3-OST-1, which makes the Bacillus expression system of particular interest for the generation of pharmaceutical grade raw heparin from nonanimal sources.;
Description
Applied Biochemistry and Biotechnology, 171, 954–962; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Department
The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
Relationships
The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Applied Biochemistry and Biotechnology; https://harc.rpi.edu/;
Access
https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1007/s12010-013-0415-8;
