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    Expression of low endotoxin 3-O-sulfotransferase in Bacillus subtilis

    Author
    Wang, Wenya; Englaender, Jacob A.; Xu, Peng; Mehta, Krunal K.; Suwan, Jiraporn; Dordick, Jonathan S.; Zhang, Fuming; Yuan, Qipeng; Linhardt, Robert J.; Koffas, Mattheos
    ORCID
    https://orcid.org/0000-0003-2219-5833
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    Other Contributors
    Date Issued
    2013-10-01
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    Expression of low endotoxin 3-O-sulfotransferase in Bacillus subtilis, W. Wang, P. Xu, J. Dordick, F. Zhang, Q. Yuan, M. Koffas, R.J. Linhardt, Applied Biochemistry and Biotechnology, 171, 954–962, 2013.
    Metadata
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    URI
    https://doi.org/10.1007/s12010-013-0415-8; https://hdl.handle.net/20.500.13015/5702
    Abstract
    A key enzyme for the biosynthesis and bioengineering of heparin, 3-O-sulfotransferase-1 (3-OST-1), was expressed and purified in Gram-positive Bacillus subtilis and Bacillus megaterium. Western blotting, protein sequence analysis, and enzyme activity measurement confirmed the expression. The enzymatic activity of 3-OST-1 expressed in Bacillus species were found to be similar to those found when expressed in Escherichia coli. The endotoxin level in 3-OST-1 from B. subtilis and B. megaterium were 10(4)-10(5)-fold lower than that of the E. coli-expressed 3-OST-1, which makes the Bacillus expression system of particular interest for the generation of pharmaceutical grade raw heparin from nonanimal sources.;
    Description
    Applied Biochemistry and Biotechnology, 171, 954–962; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Applied Biochemistry and Biotechnology; https://harc.rpi.edu/;
    Access
    https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1007/s12010-013-0415-8;
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