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Expression of low endotoxin 3-O-sulfotransferase in Bacillus subtilis

dc.contributor.authorWang, Wenya
dc.contributor.authorEnglaender, Jacob A.
dc.contributor.authorXu, Peng
dc.contributor.authorMehta, Krunal K.
dc.contributor.authorSuwan, Jiraporn
dc.contributor.authorDordick, Jonathan S.
dc.contributor.authorZhang, Fuming
dc.contributor.authorYuan, Qipeng
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorKoffas, Mattheos
dc.date2013
dc.date.accessioned2022-06-27T16:06:07Z
dc.date.available2022-06-27T16:06:07Z
dc.date.issued2013-10-01
dc.identifier.citationExpression of low endotoxin 3-O-sulfotransferase in Bacillus subtilis, W. Wang, P. Xu, J. Dordick, F. Zhang, Q. Yuan, M. Koffas, R.J. Linhardt, Applied Biochemistry and Biotechnology, 171, 954–962, 2013.
dc.identifier.issn15590291
dc.identifier.issn2732289
dc.identifier.urihttps://doi.org/10.1007/s12010-013-0415-8
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5702
dc.descriptionApplied Biochemistry and Biotechnology, 171, 954–962
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractA key enzyme for the biosynthesis and bioengineering of heparin, 3-O-sulfotransferase-1 (3-OST-1), was expressed and purified in Gram-positive Bacillus subtilis and Bacillus megaterium. Western blotting, protein sequence analysis, and enzyme activity measurement confirmed the expression. The enzymatic activity of 3-OST-1 expressed in Bacillus species were found to be similar to those found when expressed in Escherichia coli. The endotoxin level in 3-OST-1 from B. subtilis and B. megaterium were 10(4)-10(5)-fold lower than that of the E. coli-expressed 3-OST-1, which makes the Bacillus expression system of particular interest for the generation of pharmaceutical grade raw heparin from nonanimal sources.
dc.description.sponsorshipNational Institutes of Health
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1007/s12010-013-0415-8
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofApplied Biochemistry and Biotechnology
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleExpression of low endotoxin 3-O-sulfotransferase in Bacillus subtilis
dc.typeArticle
dcterms.accessRightshttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1007/s12010-013-0415-8
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1007/s12010-013-0415-8
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages954-962
rpi.description.volume171


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