Author
Suwan, Jiraporn; Torelli, Amanda; Onishi, Akihiro; Dordick, Jonathan S.; Linhardt, Robert J.
Other Contributors
Date Issued
2012-11-01
Subject
Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
Degree
Terms of Use
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Full Citation
Addressing endotoxin issues in bioengineered heparin, J. Suwan, A. Torelli, A. Onishi, J. S. Dordick, R. J. Linhardt, Biotechnology and Applied Biochemistry, 59, 420-428, 2012.
Abstract
Heparin is a widely used clinical anticoagulant that is prepared from pig intestine. A contamination of heparin in 2008 has led to a reexamination of animal-derived pharmaceuticals. A bioengineered heparin prepared by bacterial fermentation and chemical and enzymatic processing is currently under development. This study examines the challenges of reducing or removing endotoxins associated with this process that are necessary to proceed with preclinical in vivo evaluation of bioengineered heparin. The current process is assessed for endotoxin levels, and strategies are examined for endotoxin removal from polysaccharides and enzymes involved in this process.;
Description
Biotechnology and Applied Biochemistry, 59, 420-428; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Department
The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
Relationships
The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Biotechnology and Applied Biochemistry; https://harc.rpi.edu/;
Access
https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1002/bab.1042;
