Orthogonal analytical approaches to detect potential contaminants in heparin
Authors
Guerrini, Marco
Zhenqing, Zhang
Shriver, Zachary
Naggi, Annamaria
Masuko, Sayaka
Langer, Robert
Casu, Benito
Linhardt, Robert J.
Torri, Giangiacomo
Sasisekharan, Ram
ORCID
https://orcid.org/0000-0003-2219-5833
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Other Contributors
Issue Date
2009-10-06
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Degree
Terms of Use
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Full Citation
Orthogonal analytical approaches to detect potential contaminants in heparin, M. Guerrini, Z. Zhang, Z. Shriver, S. Masuko, R. Langer, B. Casu, R. J. Linhardt, G. Torri, R. Sasisekharan, Proceedings of the National Academy of Sciences USA, 106, 16956–16961, 2009.
Abstract
Heparin is a widely used anticoagulant and antithrombotic agent. Recently, a contaminant, oversulfated chondroitin sulfate (OSCS), was discovered within heparin preparations. The presence of OSCS within heparin likely led to clinical manifestations, most prevalently, hypotension and abdominal pain leading to the deaths of several dozens of patients. Given the biological effects of OSCS, one continuing item of concern is the ability for existing methods to identify other persulfonated polysaccharide compounds that would also have anticoagulant activity and would likely elicit a similar activation of the contact system. To complete a more extensive analysis of the ability for NMR and capillary electrophoresis (CE) to capture a broader array of potential contaminants within heparin, we completed a systematic study of NMR, both mono- and bidimensional, and CE to detect both various components of sidestream heparin and their persulfonated derivatives. We show that given the complexity of heparin samples, and the requirement to ensure their purity and safety, use of orthogonal analytical techniques is effective at detecting an array of potential contaminants that could be present.
Description
Proceedings of the National Academy of Sciences USA, 106, 16956–16961
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Proceedings of the National Academy of Sciences of the United States of America
https://harc.rpi.edu/
Rensselaer Polytechnic Institute, Troy, NY
Proceedings of the National Academy of Sciences of the United States of America
https://harc.rpi.edu/