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dc.contributor.authorGuerrini, Marco
dc.contributor.authorZhenqing, Zhang
dc.contributor.authorShriver, Zachary
dc.contributor.authorNaggi, Annamaria
dc.contributor.authorMasuko, Sayaka
dc.contributor.authorLanger, Robert
dc.contributor.authorCasu, Benito
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorTorri, Giangiacomo
dc.contributor.authorSasisekharan, Ram
dc.identifier.citationOrthogonal analytical approaches to detect potential contaminants in heparin, M. Guerrini, Z. Zhang, Z. Shriver, S. Masuko, R. Langer, B. Casu, R. J. Linhardt, G. Torri, R. Sasisekharan, Proceedings of the National Academy of Sciences USA, 106, 16956–16961, 2009.
dc.descriptionProceedings of the National Academy of Sciences USA, 106, 16956–16961
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractHeparin is a widely used anticoagulant and antithrombotic agent. Recently, a contaminant, oversulfated chondroitin sulfate (OSCS), was discovered within heparin preparations. The presence of OSCS within heparin likely led to clinical manifestations, most prevalently, hypotension and abdominal pain leading to the deaths of several dozens of patients. Given the biological effects of OSCS, one continuing item of concern is the ability for existing methods to identify other persulfonated polysaccharide compounds that would also have anticoagulant activity and would likely elicit a similar activation of the contact system. To complete a more extensive analysis of the ability for NMR and capillary electrophoresis (CE) to capture a broader array of potential contaminants within heparin, we completed a systematic study of NMR, both mono- and bidimensional, and CE to detect both various components of sidestream heparin and their persulfonated derivatives. We show that given the complexity of heparin samples, and the requirement to ensure their purity and safety, use of orthogonal analytical techniques is effective at detecting an array of potential contaminants that could be present.
dc.description.sponsorshipNational Heart, Lung, and Blood Institute
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of America
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleOrthogonal analytical approaches to detect potential contaminants in heparin
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)

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