Show simple item record

dc.contributor.authorSim, Joon Soo
dc.contributor.authorJun, Gyungjin
dc.contributor.authorToida, Toshihiko
dc.contributor.authorCho, So Yean
dc.contributor.authorChoi, Don Woong
dc.contributor.authorChang, Seung Yeup
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorKim, Yeong Shik
dc.identifier.citationQuantitative Analysis of Chondroitin Sulfate in Raw Materials, Ophthalmic Solutions, Soft Capsules and Liquid Preparations, J.-S. Sim, T. Toida, S. Y. Cho, D. W. Choi, S.-Y. Chang, R. J. Linhardt, Y. S. Kim, Journal of Chromatography B, 818, 133-139, 2005.
dc.descriptionJournal of Chromatography B, 818, 133-139
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractWe performed the quantitative analysis of chondroitin sulfate (CS) obtained from raw materials and various pharmaceutical preparations. To quantify CS content in raw materials and in an ophthalmic solution, each test sample and the authentic CS were first digested by chondroitinase ABC. The CS disaccharides produced were analyzed by strong anion-exchange high-performance liquid chromatography (SAX-HPLC) and CS content was quantified by calculating the total peak areas of the disaccharides derived from a CS calibration curve. In the case of soft capsules, CS was first extracted with hexane followed by phenol-chloroform to remove oil and protein ingredients. The extracted CS samples were depolymerized by chondroitinase ABC and CS content was determined. Quantitative analysis of the disaccharides derived from raw materials and an ophthalmic solution showed the CS contents (%) were 39.5+/-0.1 to 105.6+/-0.1 and 103.3+/-1.2, respectively. In case of CS analysis in soft capsules and liquid preparations, the overall recovery (%) of the spiked CS was 96.79+/-0.53-103.54+/-1.78 and 97.10+/-1.82 to 103.17+/-2.34, respectively. In conclusion, the quantitative analysis of the disaccharides produced by enzymatic digestion can be used in the direct quantitation of CS containing pharmaceutical formulations.
dc.description.sponsorshipNational Heart, Lung, and Blood Institute
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleQuantitative Analysis of Chondroitin Sulfate in Raw Materials, Ophthalmic Solutions, Soft Capsules and Liquid Preparations
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)

Files in this item


There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record