Evaluation of Counter-Ions for Electrospray Ionization Mass Spectral Analysis of Highly Sulfated Carbohydrates

Authors
Gunay, N.S.
Tadano-Aritomi, K.
Toida, T.
Ishizuka, I.
Linhardt, Robert J.
ORCID
https://orcid.org/0000-0003-2219-5833
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Other Contributors
Issue Date
2003
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Degree
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Full Citation
Evaluation of Counter-Ions for Electrospray Ionization Mass Spectral Analysis of Highly Sulfated Carbohydrates, N.S. Gunay, K. Tadano-Aritomi, T. Toida, I. Ishizuka, R.J. Linhardt, Analytical Chemistry, 75, 3226-3231, 2003.
Abstract
A systematic approach was used to evaluate the electrospray ionization mass spectral (ESI-MS) analysis of sucrose octasulfate (SOS), an important pharmaceutical agent. SOS represents a model for other suffated carbohydrates, such as heparin and glycosaminoglycan-derived oligosaccharides that also are highly sulfated and pose difficult analytical problems. A survey of ammonium counterions showed that 1 degree, 2 degrees, and 3 degrees ammonium salts of SOS gave substantial fragmentation as a result of sulfate loss. In contrast, quaternary ammonium and phosphonium salts gave excellent ESI spectra, particularly in the positive ion mode. This represents the first report of the ESI-MS analysis of sulfated carbohydrates in the positive ion mode.
Description
Analytical Chemistry, 75, 3226-3231
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Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
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The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
https://harc.rpi.edu/
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