dc.contributor.author | Chaidedgumjorn, A. | |
dc.contributor.author | Suzuki, A. | |
dc.contributor.author | Toyoda, H. | |
dc.contributor.author | Toida, T. | |
dc.contributor.author | Imanari, T. | |
dc.contributor.author | Linhardt, Robert J. | |
dc.date | 2002 | |
dc.date.accessioned | 2022-06-27T16:18:37Z | |
dc.date.available | 2022-06-27T16:18:37Z | |
dc.date.issued | 2002 | |
dc.identifier.citation | Conductivity Detection for Molecular Mass Estimation of Per-O-Sulfonated Glycosaminoglycans Seperated by High-Performance Size Exclusion Chromatography, A. Chaidedgumjorn, A. Suzuki, H. Toyoda, T. Toida, T. Imanari and R.J. Linhardt, Journal of Chromatography A, 959, 95-102. 2002. | |
dc.identifier.uri | https://doi.org/10.1016/S0021-9673(02)00446-6 | |
dc.identifier.uri | https://hdl.handle.net/20.500.13015/5821 | |
dc.description | Journal of Chromatography A, 959, 95-102 | |
dc.description | Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform. | |
dc.description.abstract | Chemically per-O-sulfonated polysaccharides, including glycosaminoglycans (GAGs) and hyaluronan oligosaccharides were analyzed using high-performance size-exclusion chromatography (HPSEC) with suppressed conductivity detection. The results were compared to those obtained by gel filtration HPLC using UV detection or fluorescence detection after the post-column reaction with 2-cyanoacetamide in strong alkaline solution. Analysis was performed on a TSKgel G3000SWXL HPSEC column in 5 mM boric acid (pH 7.0 adjusted by 10 mM NaOH). The use of conductivity detection, in the absence of any derivatization and under isocratic conditions gave a limit of detection in the picogram range. Preliminary studies suggest that this approach may be particularly useful in examining sulfonated polysaccharides and oligosaccharides having no UV chromophore, such as those prepared from O-sulfonated fucans and galactans isolated from algae. | |
dc.description.uri | https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/S0021-9673(02)00446-6 | |
dc.language | en_US | |
dc.language.iso | ENG | |
dc.relation.ispartof | The Linhardt Research Labs Online Collection | |
dc.relation.ispartof | Rensselaer Polytechnic Institute, Troy, NY | |
dc.relation.uri | https://harc.rpi.edu/ | |
dc.subject | Biology | |
dc.subject | Chemistry and chemical biology | |
dc.subject | Chemical and biological engineering | |
dc.subject | Biomedical engineering | |
dc.title | Conductivity Detection for Molecular Mass Estimation of Per-O-Sulfonated Glycosaminoglycans Seperated by High-Performance Size Exclusion Chromatography | |
dc.type | Article | |
dcterms.accessRights | https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/S0021-9673(02)00446-6 | |
dcterms.isVersionOf | https://doi.org/10.1016/S0021-9673(02)00446-6 | |
dc.rights.holder | In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/ | |
dc.creator.identifier | https://orcid.org/0000-0003-2219-5833 | |
dc.relation.department | The Linhardt Research Labs. | |
dc.relation.department | The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS) | |