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dc.contributor.authorChaidedgumjorn, A.
dc.contributor.authorSuzuki, A.
dc.contributor.authorToyoda, H.
dc.contributor.authorToida, T.
dc.contributor.authorImanari, T.
dc.contributor.authorLinhardt, Robert J.
dc.date2002
dc.date.accessioned2022-06-27T16:18:37Z
dc.date.available2022-06-27T16:18:37Z
dc.date.issued2002
dc.identifier.citationConductivity Detection for Molecular Mass Estimation of Per-O-Sulfonated Glycosaminoglycans Seperated by High-Performance Size Exclusion Chromatography, A. Chaidedgumjorn, A. Suzuki, H. Toyoda, T. Toida, T. Imanari and R.J. Linhardt, Journal of Chromatography A, 959, 95-102. 2002.
dc.identifier.urihttps://doi.org/10.1016/S0021-9673(02)00446-6
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5821
dc.descriptionJournal of Chromatography A, 959, 95-102
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractChemically per-O-sulfonated polysaccharides, including glycosaminoglycans (GAGs) and hyaluronan oligosaccharides were analyzed using high-performance size-exclusion chromatography (HPSEC) with suppressed conductivity detection. The results were compared to those obtained by gel filtration HPLC using UV detection or fluorescence detection after the post-column reaction with 2-cyanoacetamide in strong alkaline solution. Analysis was performed on a TSKgel G3000SWXL HPSEC column in 5 mM boric acid (pH 7.0 adjusted by 10 mM NaOH). The use of conductivity detection, in the absence of any derivatization and under isocratic conditions gave a limit of detection in the picogram range. Preliminary studies suggest that this approach may be particularly useful in examining sulfonated polysaccharides and oligosaccharides having no UV chromophore, such as those prepared from O-sulfonated fucans and galactans isolated from algae.
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/S0021-9673(02)00446-6
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleConductivity Detection for Molecular Mass Estimation of Per-O-Sulfonated Glycosaminoglycans Seperated by High-Performance Size Exclusion Chromatography
dc.typeArticle
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dcterms.isVersionOfhttps://doi.org/10.1016/S0021-9673(02)00446-6
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dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)


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