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    Determination of the Composition of the Oligosaccharide Phosphate Fraction of Pichia holstii NRRL Y-2448 Phosphomannan by Capillary Electrophoresis and HPLC

    Author
    Ferro, Vito; Li, Caiping; Fewings, Kym; Palermo, Maria C.; Linhardt, Robert J.; Toida, Toshihiko
    ORCID
    https://orcid.org/0000-0003-2219-5833
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    Other Contributors
    Date Issued
    2002-01-01
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    Determination of the Composition of the Oligosaccharide Phosphate Fraction of Pichia holstii NRRL Y-2448 Phosphomannan by Capillary Electrophoresis and HPLC, V. Ferro, C. Li, K. Fewings, M.C. Palermo, D. Podger, R.J. Linhardt, T. Toida, Carbohydrate Research, 337, 139-146, 2002.
    Metadata
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    URI
    https://doi.org/10.1016/S0008-6215(01)00300-7; https://hdl.handle.net/20.500.13015/5838
    Abstract
    The promising new anticancer agent, PI-88, is prepared by the sulfonation of the oligosaccharide phosphate fraction of the extracellular phosphomannan produced by the yeast Pichia (Hansenula) holstii NRRL Y-2448. The composition of the oligosaccharide phosphate fraction was determined by capillary electrophoresis (CE) with indirect UV detection using 6 mM potassium sorbate at pH 10.3 as the background electrolyte. Further confirmation of the composition was obtained by HPLC analysis of a sample dephosphorylated by treatment with alkaline phosphatase. The structure of the hexasaccharide component has been determined by isolation and NMR spectroscopic analysis of its dephosphorylated derivative. Additionally, the structure of a second, previously undetected tetrasaccharide component (a hexosamine) has been determined by isolation and NMR spectroscopic analysis of the acetate of its dephosphorylated derivative. It is demonstrated that CE is an ideal method for the quality control of the oligosaccharide phosphate fraction for use in the production of PI-88.;
    Description
    Carbohydrate Research, 337, 139-146; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Carbohydrate Research; https://harc.rpi.edu/;
    Access
    https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/S0008-6215(01)00300-7;
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