Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
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Separation and Purification of Carbohydrates, H.G. Bazin, R.J. Linhardt, in Glycoscience: Chemistry and Chemical Biology, B. Fraser-Reid, K. Tatsuta, J. Thiem, eds., Springer-Verlag, Heidelberg, Chapter 1.3, pp 64-74, 2001.
The separation and purification of carbohydrates is a broad field encompassing molecules having substantially different physical properties (see Chap. 1.2) requiring a wide variety of analytical and separation methods. This chapter is divided into three sections based on the following physical properties of the carbohydrates: unprotected/neutral; unprotected/charged; and protected (Fig. 1). This chapter will focus on preparative methods that are scalable and those that permit the preparation of sufficient carbohydrate material for biological evaluation. Several books have recently been published on micro-analytical methods in carbohydrate chemistry and glycobiology [1, 2, 3, 4, 5, 6]. These analytical methods often focus more on detection than on separation. The current chapter will only briefly describe analytical methods critical for following preparative separations. The scalability (mg to kg) of each method will be discussed together with important considerations and pertinent examples relating to each method.;
in Glycoscience: Chemistry and Chemical Biology, B. Fraser-Reid, K. Tatsuta, J. Thiem, eds., Springer-Verlag, Heidelberg, Chapter 1.3, pp 64-74; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; https://harc.rpi.edu/;