Author
LeBrun, L.A.; Linhardt, Robert J.
Other Contributors
Date Issued
2001-01-01
Subject
Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
Degree
Terms of Use
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Full Citation
Degradation of Heparan Sulfate with Heparin Lyases, L.A. LeBrun, R.J. Linhardt, in Methods in Molecular Biology, Proteoglycan Protocols: Current Methods and Applications, Vol. 171, R.V. Iozzo, ed., Humana Press, Totowa, NJ, Chap. 35, pp 353-361, 2001.
Abstract
Glycosaminoglycan (GAG), heparan sulfate (HS), and heparin are a polydisperse mixture of linear polysaccharides composed of glucosamine residues 1→ 4 linked to uronic acid residues. The major repeating unit in heparin is → 4)-α-D-N-sulfoglucosamine-6-sulfate (1? 4)-α-L-iduronic acid-2-sulfate (1?, corresponds to 75-90% of its sequence (1) (see Fig. 1A), whereas heparan sulfate consists of 50-75% ? 4)α-D-N-acetylglucosamine (1? 4)-β-glucuronic acid (1? and smaller amounts of → 4)-α-D-N-acetylglucosamine-6-sulfate (1? 4)-β-D-glucuronic acid (1? and ? 4)α-D-N-sulfoglucosamine (1? 4)-β-D-glucuronic acid (1? (see Fig. 1B). Heparin, which contains approx 2.7 sulfate groups per disaccharide unit, is more highly sulfated than HS, which contains less than one sulfate per disaccharide unit.;
Description
in Methods in Molecular Biology, Proteoglycan Protocols: Current Methods and Applications, Vol. 171, R.V. Iozzo, ed., Humana Press, Totowa, NJ, Chap. 35, pp 353-361; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Department
The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
Relationships
The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Methods in molecular biology (Clifton, N.J.); https://harc.rpi.edu/;
Access
https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1385/1-59259-209-0:353;
