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dc.contributor.authorShriver, Zachary
dc.contributor.authorRaman, Rahul
dc.contributor.authorVenkataraman, Ganesh
dc.contributor.authorDrummond, Katherine
dc.contributor.authorTurnbull, Jeremy
dc.contributor.authorToida, Toshihiko
dc.contributor.authorLinhardt, Robert
dc.contributor.authorBiemann, Klaus
dc.contributor.authorSasisekharan, Ram
dc.identifier.citationSequencing of 3-O-Sulfate Containing Heparin Decasaccharides with a Partial Antithrombin III Binding Site, Z. Shriver, R. Raman, G. Venkataraman, K. Drummond, J. Turnbull, T. Toida, R. Linhardt, K. Biemann, R. Sasisekharan, Proceedings of the National Academy of Sciences (USA), 97, 10359-10364, 2000.
dc.descriptionProceedings of the National Academy of Sciences (USA), 97, 10359-10364
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractHeparin- and heparan sulfate-like glycosaminoglycans (HLGAGs) represent an important class of molecules that interact with and modulate the activity of growth factors, enzymes, and morphogens. Of the many biological functions for this class of molecules, one of its most important functions is its interaction with antithrombin III (AT-III). AT-III binding to a specific heparin pentasaccharide sequence, containing an unusual 3-O sulfate on a N-sulfated, 6-O sulfated glucosamine, increases 1,000-fold AT-III's ability to inhibit specific proteases in the coagulation cascade. In this manner, HLGAGs play an important biological and pharmacological role in the modulation of blood clotting. Recently, a sequencing methodology was developed to further structure-function relationships of this important class of molecules. This methodology combines a property-encoded nomenclature scheme to handle the large information content (properties) of HLGAGs, with matrix-assisted laser desorption ionization MS and enzymatic and chemical degradation as experimental constraints to rapidly sequence picomole quantities of HLGAG oligosaccharides. Using the above property-encoded nomenclature-matrix-assisted laser desorption ionization approach, we found that the sequence of the decasaccharide used in this study is DeltaU(2S)H(NS,6S)I(2S)H(NS, 6S)I(2S)H(NS,6S)IH(NAc,6S)GH(NS,3S,6S) (+/-DDD4-7). We confirmed our results by using integral glycan sequencing and one-dimensional proton NMR. Furthermore, we show that this approach is flexible and is able to derive sequence information on an oligosaccharide mixture. Thus, this methodology will make possible both the analysis of other unusual sequences in HLGAGs with important biological activity as well as provide the basis for the structural analysis of these pharamacologically important group of heparin/heparan sulfates.
dc.description.sponsorshipNational Institute of General Medical Sciences
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of America
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleSequencing of 3-O-Sulfate Containing Heparin Decasaccharides with a Partial Antithrombin III Binding Site
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)

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