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dc.contributor.authorYang, Hyun Ok
dc.contributor.authorGunay, Nur Sibel
dc.contributor.authorToida, Toshihiko
dc.contributor.authorKuberan, Balagurunathan
dc.contributor.authorYu, Guangli
dc.contributor.authorKim, Yeong Shik
dc.contributor.authorLinhardt, Robert J.
dc.date2000
dc.date.accessioned2022-06-27T16:20:27Z
dc.date.available2022-06-27T16:20:27Z
dc.date.issued2000-01-01
dc.identifier.citationPreparation and Structural Determination of Dermatan Sulfate-Derived Oligosaccharides, H.O. Yang, N.S. Gunay, T. Toida, B. Kuberan, G. Yu, Y.S. Kim, R.J. Linhardt, Glycobiology, 10, 1033-1040, 2000.
dc.identifier.issn9596658
dc.identifier.urihttps://doi.org/10.1093/glycob/10.10.1033
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5867
dc.descriptionGlycobiology, 10, 1033-1040
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractEight oligosaccharides were prepared from dermatan sulfate (DS) and their structures were elucidated. Porcine intestinal mucosal DS was subjected to controlled depolymerization using chondroitin ABC lyase (chondroitinase ABC). The oligosaccharide mixture formed was fractionated by low-pressure gel permeation chromatography (GPC). Size uniform mixtures of disaccharides, tetrasaccharides, hexasaccharides, octasaccharides, decasaccharides, and dodecasaccharides were obtained. Each size-fractionated mixture was then purified on the basis of charge by repetitive semi-preparative strong-anion-exchange (SAX) high-performance liquid chromatography (HPLC). This approach has led to the isolation of six homogeneous oligosaccharides. The size of the oligosaccharides were determined using GPC-HPLC. Treatment of tetrasaccharide and hexasaccharide fragments with Hg(OAc)2 afforded trisaccharide and pentasaccharide products, respectively. The purity of the oligosaccharides obtained was confirmed by analytical SAX-HPLC, and capillary electrophoresis (CE). The molecular mass and degree of sulfation of the eight purified oligosaccharides were elucidated using electrospray ionization (ESI) mass spectrometry and their structures were established with high field nuclear magnetic resonance (NMR) spectroscopy. These DS-oligosaccharides are currently being used to study for interaction of the DS with biologically important proteins.
dc.description.sponsorshipNational Heart, Lung, and Blood Institute
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1093/glycob/10.10.1033
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofGlycobiology
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titlePreparation and Structural Determination of Dermatan Sulfate-Derived Oligosaccharides
dc.typeArticle
dcterms.accessRightshttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1093/glycob/10.10.1033
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1093/glycob/10.10.1033
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages1033-1040
rpi.description.volume10


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