Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
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Analysis of fluorescently labeled oligosaccharides by capillary electrophoresis and electrospray ionization mass spectrometryH. G. Bazin, M.W. Wolff, R.J. Linhardt, Biotechnology Techniques,13,797-801, 1999.
Neutral oligosaccharides were fluorescently conjugated with 7-amino-1, 3-naphthalenedisulfonic acid. A mixture of fluorescently labeled chitobiose, chitotriose, and chitotetrose were successfully separated by preparative capillary electrophoresis (CE) and the individual components characterized by electrospray ionization-mass spectrometry (ESI-MS). By combining fluorescent labeling with CE, the use of highly specific exoglycosidases and ESI-MS, a more structurally complex N-linked glycan was analyzed.;
Biotechnology Techniques,13,797-801; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Biotechnology Techniques; https://harc.rpi.edu/;