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dc.contributor.authorCapila, Ishan
dc.contributor.authorVandernoot, Victoria A.
dc.contributor.authorMealy, Tanya R.
dc.contributor.authorSeaton, Barbara A.
dc.contributor.authorLinhardt, Robert J.
dc.date1999
dc.date.accessioned2022-06-27T16:21:12Z
dc.date.available2022-06-27T16:21:12Z
dc.date.issued1999-03-12
dc.identifier.citationInteraction of Heparin with Annexin V, I. Capila, V. A. VanderNoot,T. R. Mealy, B. A. Seaton, RJ. Linhardt, FEBS Letters, 446,327-330, 1999.
dc.identifier.issn145793
dc.identifier.urihttps://doi.org/10.1016/S0014-5793(99)00245-8
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5882
dc.descriptionFEBS Letters, 446, 327-330
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractThe energetics and kinetics of the interaction of heparin with the Ca2+ and phospholipid binding protein annexin V, was examined and the minimum oligosaccharide sequence within heparin that binds annexin V was identified. Affinity chromatography studies confirmed the Ca2+ dependence of this binding interaction. Analysis of the data obtained from surface plasmon resonance afforded a Kd of approximately 21 nM for the interaction of annexin V with end-chain immobilized heparin and a Kd of approximately 49 nM for the interaction with end-chain immobilized heparan sulfate. Isothermal titration calorimetry showed the minimum annexin V binding oligosaccharide sequence within heparin corresponds to an octasaccharide sequence. The Kd of a heparin octasaccharide binding to annexin V was approximately 1 microM with a binding stoichiometry of 1:1.
dc.description.sponsorshipNational Institutes of Health
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofFEBS Letters
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleInteraction of Heparin with Annexin V
dc.typeArticle
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1016/S0014-5793(99)00245-8
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages327-330
rpi.description.volume446


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