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dc.contributor.authorWu, S.J.
dc.contributor.authorChun, M.W.
dc.contributor.authorShin, K.H.
dc.contributor.authorToida, T.
dc.contributor.authorPark, Y.
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorKim, Y.S.
dc.date1998
dc.date.accessioned2022-06-27T16:21:51Z
dc.date.available2022-06-27T16:21:51Z
dc.date.issued1998
dc.identifier.citationChemical Oversulfation and Anticoagulant Activity of Acharan Sulfate,S.J. Wu, M. W. Chun, K. H. Shin, T. Toida, Y. Park, R.J. Linhardt, Y.S.Kim, Thrombosis Research, 92, 273-281, 1998.
dc.identifier.urihttps://doi.org/10.1016/s0049-3848(98)00146-7
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5893
dc.descriptionThrombosis Research, 92, 273-281
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractcharan sulfate is a glycosaminoglycan prepared from the giant African snail, Achatina fulica. This polysaccharide has a repeating disaccharide structure of -->4)-2-deoxy-2-acetamido-alpha-D-glucopyranose (1-->4)-2-sulfo-alpha-L-idopyranosyluronic acid (1-->). Its structure is related to heparin and heparan sulfate but is distinctly different from all known members of these classes of glycosaminoglycans. Because of its structural similarities to heparin, chemically modified acharan sulfate was studied to understand the chemical structure effected its anticoagulant activity. After de-N-acetylation, acharan sulfate was N-sulfonated using either chlorosulfonic acid-pyridine or sulfur trioxide-trimethylamine complex. The sulfate level in these products ranged from 22 to 24%(w/w), significantly less than that of heparin at 36%. The molecular weight of both N-sulfoacharan sulfates were comparable with that of heparin. In vitro anticoagulant activity assays showed that N-sulfoacharan sulfate derivatives were moderately active for the inhibition of thrombin and neither product showed any measurable anti-factor Xa activity. The differences in the activities of N-sulfoacharan sulfates produced by these two methods are probably ascribable to a small level of concomitant O-sulfonation obtained when using chlorosulfonic acid-pyridine.
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/s0049-3848(98)00146-7
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleChemical Oversulfation and Anticoagulant Activity of Acharan Sulfate
dc.typeArticle
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dcterms.isVersionOfhttps://doi.org/10.1016/s0049-3848(98)00146-7
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dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)


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