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dc.contributor.authorWu, X.
dc.contributor.authorLinhardt, Robert J.
dc.date1998
dc.date.accessioned2022-06-27T16:21:52Z
dc.date.available2022-06-27T16:21:52Z
dc.date.issued1998
dc.identifier.citationCapillary Affinity Chromatography and Affinity Capillary Electrophoresisof Heparin Binding Proteins, X. Wu, R.J. Linhardt, Electrophoresis,19,2650-2653, 1998.
dc.identifier.urihttps://doi.org/10.1002/elps.1150191514
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5894
dc.descriptionElectrophoresis, 19, 2650-2653
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractA new approach for separation, capillary affinity chromatography, is introduced for studying the interaction of heparin with antithrombin III and secretory leukocyte proteinase inhibitor. Heparin is covalently immobilized on the surface of an etched capillary through a silane spacer. The proteins are injected into the heparinized capillary, bound to the heparin, washed with buffer, eluted with sodium chloride in the same buffer using a pressure injection mode and eluting protein detected by absorbance. The resulting affinity separation is similar to that obtained from traditional affinity chromatography. The quantity of loaded protein in capillary affinity chromatography is at the nanogram level, offering an improvement over the milligram levels required for standard affinity chromatographic methods.
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1002/elps.1150191514
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleCapillary Affinity Chromatography and Affinity Capillary Electrophoresisof Heparin Binding Proteins
dc.typeArticle
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dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)


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