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dc.contributor.authorKim, Yeong Shik
dc.contributor.authorAhn, Mi Young
dc.contributor.authorWu, Song Ji
dc.contributor.authorKim, Dong Hyun
dc.contributor.authorToida, Toshihiko
dc.contributor.authorTeesch, Lynn M.
dc.contributor.authorPark, Youmie
dc.contributor.authorYu, Guyong
dc.contributor.authorLin, Jihon
dc.contributor.authorLinhardt, Robert J.
dc.date1998
dc.date.accessioned2022-06-27T16:22:18Z
dc.date.available2022-06-27T16:22:18Z
dc.date.issued1998-01-01
dc.identifier.citationDetermination of the Structure of Oligosaccharides Prepared fromAcharan Sulfate, Y.S. Kim, M.Y. Ahn, S.J. Wu, D.-H. Kim, T. Toida, L.M.Teesch, Y. Park, G. Yu, J. Lin, R.J. Linhardt, Glycobiology, 8,869-877, 1998.
dc.identifier.issn9596658
dc.identifier.urihttps://doi.org/10.1093/glycob/8.9.869
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5900
dc.descriptionGlycobiology, 8, 869-877
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractThe fine structure of acharan sulfate, a recently discovered glycosaminoglycan isolated from Achatina fulica , was examined. This glycosaminoglycan has a major disaccharide repeating unit of -->4)-alpha-D-GlcNpAc(1-->4)-alpha-L-IdoAp2S(1--> (where GlcNpAc is N -acetylglucosamine, IdoAp is iduronic acid, and S is sulfate) making it structurally related to both heparin and heparan sulfate. Using heparin lyases prepared from Flavobacterium heparinum and a newly isolated heparinase from Bacteroides stercoris , the controlled enzymatic depolymerization of acharan sulfate was undertaken to prepare a mixture of oligosaccharides. Fractionation of this mixture of oligosaccharides by strong-anion-exchange high performance liquid chromatography afforded oligosaccharides that capillary electrophoresis established were sufficiently pure for structural characterization. Electrospray ionization mass spectrometry identified two series of oligosaccharides, one derived from acharan sulfate's major repeating unit and a second minor group of undersulfated oligosaccharides. Proton nuclear magnetic resonance spectroscopy established the structure of these two classes of oligosaccharides to be DeltaUAp2S(1-->[4)-alpha-D-GlcNpAc(1-->4)-alpha-L-IdoAp2S (1-->]n4)- D-GlcNpAcalpha,beta (where n = 0,1,2,3 and DeltaUAp is 4-deoxy-alpha-L- threo -hex-4-enopyranosyluronic acid) and DeltaUAp(1-->[4)- alpha-D-GlcNpAc(1-->4)-alpha-L-IdoAp2S(1-->]m-D-GlcNpAcal pha,beta (where m = 1,2,3). These results suggest the presence of minor sequence variants in acharan sulfate containing unsulfated iduronic acid having the structure -->4)-alpha-D-GlcNpAc(1-->4)-alpha-L-IdoAp(1-->.
dc.description.sponsorshipNational Science Foundation
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofGlycobiology
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleDetermination of the Structure of Oligosaccharides Prepared from Acharan Sulfate
dc.typeArticle
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1093/glycob/8.9.869
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages869-877
rpi.description.volume8


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