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Detection of Glycosaminoglycans as a Copper (II) Complex in HPLC

dc.contributor.authorToida, T.
dc.contributor.authorShima, M.
dc.contributor.authorAzumaya, S.
dc.contributor.authorMaruyama, T.
dc.contributor.authorToyoda, H.
dc.contributor.authorImanari, T.
dc.contributor.authorLinhardt, Robert J.
dc.date1997
dc.date.accessioned2022-06-27T16:22:19Z
dc.date.available2022-06-27T16:22:19Z
dc.date.issued1997
dc.identifier.citationDetection of Glycosaminoglycans as a Copper (II) Complex in HPLC,T. Toida, M. Shima, S. Azumaya, T. Maruyama, H. Toyoda, T. Imanari, R.J.Linhardt, Journal of Chromatography, 787, 266-270, 1997.
dc.identifier.urihttps://doi.org/10.1016/S0021-9673(97)00658-4
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5907
dc.descriptionJournal of Chromatography, 787, 266-270
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractGlycosaminoglycans including heparin, heparan sulfate, chondroitin sulfate, dermatan sulfate and hyaluronan were analyzed by high-performance gel filtration chromatography. Detection was achieved at 240 nm based on the formation of copper(II) complex in copper sulfate solution at low pH. Detection of the copper(II)-heparin complex is sensitive, permitting the analysis of as little as 10(-7) g. This method was also successfully applied to the analysis of the chemically derivatized glycosaminoglycans (de-N-acetylated and/or oversulfated chondroitin sulfate) that cannot be depolymerized by enzymes such as heparin and chondroitin lyases.
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dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleDetection of Glycosaminoglycans as a Copper (II) Complex in HPLC
dc.typeArticle
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dcterms.isVersionOfhttps://doi.org/10.1016/S0021-9673(97)00658-4
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dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)


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