dc.contributor.author | Toida, T. | |
dc.contributor.author | Shima, M. | |
dc.contributor.author | Azumaya, S. | |
dc.contributor.author | Maruyama, T. | |
dc.contributor.author | Toyoda, H. | |
dc.contributor.author | Imanari, T. | |
dc.contributor.author | Linhardt, Robert J. | |
dc.date | 1997 | |
dc.date.accessioned | 2022-06-27T16:22:19Z | |
dc.date.available | 2022-06-27T16:22:19Z | |
dc.date.issued | 1997 | |
dc.identifier.citation | Detection of Glycosaminoglycans as a Copper (II) Complex in HPLC,T. Toida, M. Shima, S. Azumaya, T. Maruyama, H. Toyoda, T. Imanari, R.J.Linhardt, Journal of Chromatography, 787, 266-270, 1997. | |
dc.identifier.uri | https://doi.org/10.1016/S0021-9673(97)00658-4 | |
dc.identifier.uri | https://hdl.handle.net/20.500.13015/5907 | |
dc.description | Journal of Chromatography, 787, 266-270 | |
dc.description | Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform. | |
dc.description.abstract | Glycosaminoglycans including heparin, heparan sulfate, chondroitin sulfate, dermatan sulfate and hyaluronan were analyzed by high-performance gel filtration chromatography. Detection was achieved at 240 nm based on the formation of copper(II) complex in copper sulfate solution at low pH. Detection of the copper(II)-heparin complex is sensitive, permitting the analysis of as little as 10(-7) g. This method was also successfully applied to the analysis of the chemically derivatized glycosaminoglycans (de-N-acetylated and/or oversulfated chondroitin sulfate) that cannot be depolymerized by enzymes such as heparin and chondroitin lyases. | |
dc.description.uri | https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/S0021-9673(97)00658-4 | |
dc.language | en_US | |
dc.language.iso | ENG | |
dc.relation.ispartof | The Linhardt Research Labs Online Collection | |
dc.relation.ispartof | Rensselaer Polytechnic Institute, Troy, NY | |
dc.relation.uri | https://harc.rpi.edu/ | |
dc.subject | Biology | |
dc.subject | Chemistry and chemical biology | |
dc.subject | Chemical and biological engineering | |
dc.subject | Biomedical engineering | |
dc.title | Detection of Glycosaminoglycans as a Copper (II) Complex in HPLC | |
dc.type | Article | |
dcterms.accessRights | https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/S0021-9673(97)00658-4 | |
dcterms.isVersionOf | https://doi.org/10.1016/S0021-9673(97)00658-4 | |
dc.rights.holder | In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/ | |
dc.creator.identifier | https://orcid.org/0000-0003-2219-5833 | |
dc.relation.department | The Linhardt Research Labs. | |
dc.relation.department | The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS) | |