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dc.contributor.authorToida, Toshihiko
dc.contributor.authorHuang, Yong
dc.contributor.authorWashio, Yukio
dc.contributor.authorMaruyama, Tomohiro
dc.contributor.authorToyoda, Hidenao
dc.contributor.authorImanari, Toshio
dc.contributor.authorLinhardt, Robert J.
dc.date1997
dc.date.accessioned2022-06-27T16:22:19Z
dc.date.available2022-06-27T16:22:19Z
dc.date.issued1997-09-05
dc.identifier.citationChemical Microdetermination of Heparin in Plasma T. Toida, Y.Huang, T. Maruyama, H. Toyoda, T. Imanari, R.J. Linhardt, AnalyticalBiochemistry, 251, 219-226, 1997.
dc.identifier.issn32697
dc.identifier.urihttps://doi.org/10.1006/abio.1997.2277
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5912
dc.descriptionAnalytical Biochemistry, 251, 219-226
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractA new, simple, and highly sensitive method for the determination of heparin has been established. Heparin was first converted into unsaturated disaccharides through the action of heparin lyases I, II, and III. A major trisulfated unsaturated disaccharide product results, consistent with structural analysis of a number of pharmaceutical heparins using one- and two-dimensional 1H NMR spectroscopy. This disaccharide was analyzed by HPLC using fluorometric postcolumn derivatization. The correlation between the amount of this trisulfated unsaturated disaccharide and anticoagulant activity of heparin as measured by anti-IIa was determined. The analysis of these pharmaceutical heparins showed a linear correlation between both HPLC and bioassay. This HPLC method was then applied to a pharmacokinetic study of heparin intravenously administered to rabbits.
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1006/abio.1997.2277
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofAnalytical Biochemistry
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleChemical Microdetermination of Heparin in Plasma
dc.typeArticle
dcterms.accessRightshttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1006/abio.1997.2277
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dcterms.isVersionOfhttps://doi.org/10.1006/abio.1997.2277
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages219-226
rpi.description.volume251


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