Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
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Analysis of the Structure of Heparin and Heparan Sulfate by HighResolution Separation of Oligosaccharides, R.J. Linhardt, T. Toida, A.E. Smith, R. E. Hileman, in A Laboratory Guide to Glycoconjugate Analysis,J. T. Gallagher, P. Jackson, eds., Birkhauser Verlag AG, Basel, 1997, Chapter10,pp 183-197.
Heparin and heparan sulfate are mixtures of highly sulfated linear polysaccharides having a molecular weight (MW) ranging from 5000 to 40,000 with an MW avg of 10,000 to 25,000 (1). They are members of a family of molecules called glycosaminoglycans (GAGs). Both heparin and heparan sulfate are biosynthesized as a repeating →4)α-D-GlcNpAc(1→4)-β-D-GlcAp(1→ disaccharide sequence that undergoes partial N-deacetylation followed by N-sulfation of the newly exposed amino groups, partial C-5 epimerization of D-GlcAp to L-IdoAp and O-sulfation. A single disaccharide sequence, [→4)α-D-GlcNpS6S-(1→4)-a-L-IdoAp2S (1→], accounts for 80 to 90% of the heparin polymer. Minor sequences contain (β-D-GlcAp residues, reduced sulfation as well as N-acetylation. Heparan sulfate is structurally similar to heparin but has substantially lower sulfation. Heparan sulfate consists primarily of unsulfated disaccharide [→4)α-D-GlcNpS-(1→4)-β-D-GlcAp(1 →] and monosulfated disaccharides [→4)α-D-GlcNpS-(1→4)-β-D-GlcAp(1→] and [→4)α-D-GlcNpAc6S(1→4)-β-D-GlcAp(1 →].;
in A Laboratory Guide to Glycoconjugate Analysis, J. T. Gallagher, P. Jackson, eds., Birkhauser Verlag AG, Basel, 1997, Chapter 10, pp 183-197; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; https://harc.rpi.edu/;