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dc.contributor.authorToida, T.E.
dc.contributor.authorLinhardt, Robert J.
dc.date1996
dc.date.accessioned2022-06-27T17:13:46Z
dc.date.available2022-06-27T17:13:46Z
dc.date.issued1996
dc.identifier.citationDetection of Glycosaminoglycans as a Copper (II) Complex in Capillary Electrophoresis, T. E. Toida, R.J. Linhardt, Electrophoresis, 17, 341-346, 1996.
dc.identifier.urihttps://doi.org/10.1002/elps.1150170209
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5937
dc.descriptionElectrophoresis, 17, 341-346
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractGlycosaminoglycans including heparin, heparan sulfate, chondroitin sulfate, dermatan sulfate and hyaluronic acid were analyzed by reversed polarity capillary electrophoresis. Detection was achieved at 240 nm based on the formation of a copper (II) complex in copper sulfate solution at low pH. Glycosaminoglycans having a high ratio of iduronic acid to glucuronic acid, as well as ones having lower molecular weight, gave the highest detection sensitivity using this method. Detection of the copper (II)-heparin complex is extremely sensitive, permitting the analysis of as little as 10−9 g. This method was also successfully applied to the analysis of heparin oligosaccharides that lacked a chromophore prepared from heparin using controlled, low pH nitrous acid depolymerization.
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1002/elps.1150170209
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleDetection of Glycosaminoglycans as a Copper (II) Complex in Capillary Electrophoresis
dc.typeArticle
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dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)


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