Modified Assay for the Measurement of Biotin in the Presence of Heparin

Authors
Nadkarni, V.D.
Linhardt, Robert J.
ORCID
https://orcid.org/0000-0003-2219-5833
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Other Contributors
Issue Date
1995-01-01
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Degree
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Full Citation
Modified Assay for the Measurement of Biotin in the Presence of Heparin, V. D. Nadkarni, R. J. Linhardt, Analytical Biochemistry, 227, 402-404, 1995.
Abstract
Tween 20 is frequently used in blotting analysis of proteins and nucleic acids to decrease background staining due to nonspecific binding of antibodies to various membranes. Inclusion of 0.1% (w/v) Tween 20 in the washing buffer (Buffer 1) significantly decreased the chemiluminescence signals of both LMW-EGF and HMW-EGFs (data not shown). This result indicates that the binding of proteins to PVDF membranes is susceptible to the detergent even after fixation with formaldehyde.
Description
Analytical Biochemistry, 227, 402-404
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Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Analytical Biochemistry
https://harc.rpi.edu/
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