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dc.contributor.authorNadkarni, V.D.
dc.contributor.authorLinhardt, Robert J.
dc.date1995
dc.date.accessioned2022-06-27T17:13:46Z
dc.date.available2022-06-27T17:13:46Z
dc.date.issued1995-01-01
dc.identifier.citationModified Assay for the Measurement of Biotin in the Presence of Heparin, V. D. Nadkarni, R. J. Linhardt, Analytical Biochemistry, 227, 402-404, 1995.
dc.identifier.issn32697
dc.identifier.urihttps://doi.org/10.1006/abio.1995.1303
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5950
dc.descriptionAnalytical Biochemistry, 227, 402-404
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractTween 20 is frequently used in blotting analysis of proteins and nucleic acids to decrease background staining due to nonspecific binding of antibodies to various membranes. Inclusion of 0.1% (w/v) Tween 20 in the washing buffer (Buffer 1) significantly decreased the chemiluminescence signals of both LMW-EGF and HMW-EGFs (data not shown). This result indicates that the binding of proteins to PVDF membranes is susceptible to the detergent even after fixation with formaldehyde.
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1006/abio.1995.1303
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofAnalytical Biochemistry
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleModified Assay for the Measurement of Biotin in the Presence of Heparin
dc.typeArticle
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dcterms.isVersionOfhttps://doi.org/10.1006/abio.1995.1303
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages402-404
rpi.description.volume227


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