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    Molecular Weight of Heparin Using 13C Nuclear Magnetic Resonance Spectroscopy

    Author
    Desai, Umesh R.; Linhardt, Robert J.
    ORCID
    https://orcid.org/0000-0003-2219-5833
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    Other Contributors
    Date Issued
    1995-01-01
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    Molecular Weight of Heparin Using 13C Nuclear Magnetic Resonance Spectroscopy, U.R. Desai, R.J. Linhardt, Journal of Pharmaceutical Science, 84, 212-215, 1995.
    Metadata
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    URI
    https://doi.org/10.1002/jps.2600840218; https://hdl.handle.net/20.500.13015/5952
    Abstract
    Heparin is a polydisperse, heterogeneous polysaccharide that has been used as an anticoagulant for the past 50 years. The molecular weight determination of this important drug has traditionally relied on gel permeation chromatography, which requires the use of well-defined molecular weight standards that are not easily obtained. We have investigated the use of 13C-NMR spectroscopy for measuring the number average molecular weight of heparin. The signal intensities of the reducing end and internal anomeric carbons, having distinctive chemical shifts in the 13C-NMR spectrum, were used to determine the molecular weight. Distortionless enhancement polarization transfer was found to provide a better quantitation of signal intensities of anomeric carbons than broad band decoupling or selective decoupling of anomeric protons. Signal averaging over 300,000 transients, requiring approximately 48 h on a 360 MHz NMR spectrometer, resulted in the measurement of the number average molecular weight (approximately 10,000 Da) of heparin. 13C-NMR spectroscopy does not require the use of difficult to obtain molecular weight standards and thus is particularly well-suited for workers in the pharmaceutical industry.;
    Description
    Journal of Pharmaceutical Science, 84, 212-215; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Journal of Pharmaceutical Sciences; https://harc.rpi.edu/;
    Access
    https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1002/jps.2600840218;
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