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dc.contributor.authorKim, Y.S.
dc.contributor.authorLiu, J.
dc.contributor.authorHan, X.J.
dc.contributor.authorPervin, A.
dc.contributor.authorLinhardt, Robert J.
dc.identifier.citationAnalysis of Fluorescently Labeled Sugars by Reversed-Phase Ion-Pairing High Performance Liquid Chromatography, Y. S. Kim, J. Liu, X.J. Han, A. Pervin, R. J. Linhardt, Journal of Chromatographic Science, 33, 162-167 1995.
dc.descriptionJournal of Chromatographic Science, 33, 162-167
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractReducing sugars, including monosaccharides, disaccharides, and a trisaccharide, are derivatized by reductive amination with 7-amino-1,3-naphthalene disulfonic acid. Reversed-phase ion-pairing high-performance liquid chromatography is then used to separate these visibly fluorescent, charged conjugates. Isocratic elution with triethylamine-acetic acid from a phenyl column, a C18 column, and C18 and phenyl columns in series gives good separations of a mixture of monosaccharides and a mixture of disaccharides and trisaccharides. Resolution of certain monosaccharides is enhanced by replacing triethylamine with a chiral amine and using gradient elution. Further enhancement of resolution is achieved by adding phenylboronic acid, an agent capable of complexing with the vicinal diol functionality present in many sugars. The trimethylamine-acetic acid eluant permits detection by either ultraviolet absorbance or fluorescence, and the addition of a chiral ion-pairing agent or a phenylboronic acid complexing agent necessitates fluorescence detection. A reversible Schiff base form of the fluorescent sugar conjugate is prepared; it is sufficiently stable to perform fractionations but sufficiently unstable to be converted to a fluorescent label and reducing sugar.
dc.description.sponsorshipNational Institutes of Health
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofJournal of chromatographic science
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleAnalysis of Fluorescently Labeled Sugars by Reversed-Phase Ion-Pairing High Performance Liquid Chromatography
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)

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