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dc.contributor.authorNadkarni, V.D.
dc.contributor.authorPervin , A.
dc.contributor.authorLinhardt, Robert J.
dc.date1994
dc.date.accessioned2022-06-27T17:14:13Z
dc.date.available2022-06-27T17:14:13Z
dc.date.issued1994-01-01
dc.identifier.citationDirectional Immobilization of Heparin to Beaded Supports, V. D. Nadkarni, A. Pervin , R. J. Linhardt, Analytical Biochemistry, 222, 59-67, 1994.
dc.identifier.issn32697
dc.identifier.urihttps://doi.org/10.1006/abio.1994.1454
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5960
dc.descriptionAnalytical Biochemistry, 222, 59-67
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractHeparin was immobilized in a defined orientation on Sepharose, agarose, and polyacrylamide supports by coupling through its reducing end. This is expected to mimic the attachment of heparin to the protein core in the naturally occurring proteoglycan and impart better ligand binding efficiency by exposing all the binding sites available in the naturally occurring heparin. The coupling chemistry was accomplished by modifying heparin at its reducing end to introduce reactive functionality that can react with appropriately functionalized supports. Three reducing end modified heparins were synthesized and characterized: 2,6-diaminopyridinyl heparin, containing a reactive amino group at the reducing end; ω-hydrazido-adipyl-azo heparin, containing a hydrazido group at the reducing end; and heparin lactone, containing a reactive ester functionality at the reducing end. These heparin derivatives were then reacted with the supports to give directionally immobilized heparin using different coupling chemistries: coupling of reducing end modified heparins to amine-containing supports (i.e., ω-aminohexyl Sepharose and omega-aminobutyl agarose), hydrazide-containing supports (i.e., Emphaze hydrazide), and activated carboxy-containing supports (i.e., activated 6-aminohexanoic acid Sepharose, Emphaze azlactone). The heparinized matrices were prepared and analyzed for their heparin content and protamine binding capacity.
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1006/abio.1994.1454
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofAnalytical Biochemistry
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleDirectional Immobilization of Heparin to Beaded Supports
dc.typeArticle
dcterms.accessRightshttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1006/abio.1994.1454
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dcterms.isVersionOfhttps://doi.org/10.1006/abio.1994.1454
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages59-67
rpi.description.volume222


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