• Login
    View Item 
    •   DSpace@RPI Home
    • The Linhardt Research Labs
    • Linhardt Research Labs Papers
    • View Item
    •   DSpace@RPI Home
    • The Linhardt Research Labs
    • Linhardt Research Labs Papers
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Analysis of Glycosaminoglycans with Polysaccharide Lyases

    Author
    Linhardt, Robert J.
    ORCID
    https://orcid.org/0000-0003-2219-5833
    Thumbnail
    Other Contributors
    Date Issued
    2001-01-01
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    Analysis of Glycosaminoglycans with Polysaccharide Lyases R. J. Linhardt, in Current Protocols in Molecular Biology, Analysis of Glycoconjugates, A. Varki (ed.), V 2, 17.13.17-17.13.32, Wiley Interscience, 1994.
    Metadata
    Show full item record
    URI
    https://doi.org/10.1002/0471142727.mb1713bs48; https://hdl.handle.net/20.500.13015/5970
    Abstract
    Polysaccharide lyases are a class of enzymes useful for analysis of glycosaminoglycans (GAGs) and the glycosaminoglycan component of proteoglycans (PGs). These enzymes cleave specific glycosidic linkages present in acidic polysaccharides and result in depolymerization. The lyases are derived from a wide variety of pathogenic and nonpathogenic bacteria and fungi. This class of enzymes includes heparin lyases (heparinases), heparan sulfate lyases (heparanases or heparitinases), chondroitin lyases (chondroitinases), and hyaluronate lyases (hyaluronidases), all of which are described in this unit. Two protocols describe depolymerization of GAGs, and two support protocols describe assays to confirm and quantitate the activity of heparin and chondroitin ABC lyases.;
    Description
    in Current Protocols in Molecular Biology, Analysis of Glycoconjugates, A. Varki (ed.), V 2, 17.13.17-17.13.32, Wiley Interscience,; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]; https://harc.rpi.edu/;
    Access
    Collections
    • Linhardt Research Labs Papers

    Browse

    All of DSpace@RPICommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    Login

    DSpace software copyright © 2002-2022  DuraSpace
    Contact Us | Send Feedback
    DSpace Express is a service operated by 
    Atmire NV