| dc.contributor.author | Linhardt, Robert J. | |
| dc.date | 1994 | |
| dc.date.accessioned | 2022-06-27T17:14:37Z | |
| dc.date.available | 2022-06-27T17:14:37Z | |
| dc.date.issued | 2001-01-01 | |
| dc.identifier.citation | Analysis of Glycosaminoglycans with Polysaccharide Lyases R. J. Linhardt, in Current Protocols in Molecular Biology, Analysis of Glycoconjugates, A. Varki (ed.), V 2, 17.13.17-17.13.32, Wiley Interscience, 1994. | |
| dc.identifier.issn | 19343647 | |
| dc.identifier.uri | https://doi.org/10.1002/0471142727.mb1713bs48 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.13015/5970 | |
| dc.description | in Current Protocols in Molecular Biology, Analysis of Glycoconjugates, A. Varki (ed.), V 2, 17.13.17-17.13.32, Wiley Interscience, | |
| dc.description | Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform. | |
| dc.description.abstract | Polysaccharide lyases are a class of enzymes useful for analysis of glycosaminoglycans (GAGs) and the glycosaminoglycan component of proteoglycans (PGs). These enzymes cleave specific glycosidic linkages present in acidic polysaccharides and result in depolymerization. The lyases are derived from a wide variety of pathogenic and nonpathogenic bacteria and fungi. This class of enzymes includes heparin lyases (heparinases), heparan sulfate lyases (heparanases or heparitinases), chondroitin lyases (chondroitinases), and hyaluronate lyases (hyaluronidases), all of which are described in this unit. Two protocols describe depolymerization of GAGs, and two support protocols describe assays to confirm and quantitate the activity of heparin and chondroitin ABC lyases. | |
| dc.language | en_US | |
| dc.language.iso | ENG | |
| dc.relation.ispartof | The Linhardt Research Labs Online Collection | |
| dc.relation.ispartof | Rensselaer Polytechnic Institute, Troy, NY | |
| dc.relation.ispartof | Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.] | |
| dc.relation.uri | https://harc.rpi.edu/ | |
| dc.subject | Biology | |
| dc.subject | Chemistry and chemical biology | |
| dc.subject | Chemical and biological engineering | |
| dc.subject | Biomedical engineering | |
| dc.title | Analysis of Glycosaminoglycans with Polysaccharide Lyases | |
| dc.type | Article | |
| dcterms.isPartOf | Journal | |
| dcterms.isVersionOf | https://doi.org/10.1002/0471142727.mb1713bs48 | |
| dc.rights.holder | In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/ | |
| dc.creator.identifier | https://orcid.org/0000-0003-2219-5833 | |
| dc.relation.department | The Linhardt Research Labs. | |
| dc.relation.department | The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS) | |
| rpi.description.volume | Chapter 17 | |
