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dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorLiu, J.
dc.contributor.authorHan, X.-J.
dc.identifier.citationMapping and Sequencing of Oligosaccharides by Electrophoresis R.J. Linhardt, J. Liu, X. -J. Han, Trends in Glycoscience and Glycotechnology, 5, 181-192 (1993).
dc.descriptionTrends in Glycoscience and Glycotechnology, 5, 181-192
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractThe analysis of oligosaccharides and polysaccharides takes on increased importance with the discovery of each new biological activity attributable to this important class of biopolymers. Our understanding of other biopolymers, pro-teins and nucleic acids, has relied heavily on electrophoresis. This minireview describes the application of capillary electro-phoresis and gradient polyacrylamide gel electrophoresis to saccharide compositional analysis, oligosaccharide mapping and oligosaccharide and polysaccharide sequencing. These ap-proaches are applicable to both acidic polysaccharides (i.e., glycosaminoglycans) and neutral, glycoprotein-derived oligo-saccharides. Separation by electrophoresis can be driven by the inherent charge of a carbohydrate, through the complexation of a carbohydrate with a charged molecule, or through derivati-zation reactions to prepare charged carbohydrate conjugates. New approaches including fluorescence labeling provide the high detection sensitivity required for microanalysis. The high resolution necessary for oligosaccharide analysis can be achieved using capillary electrophoresis or two-dimensional gel electrophoresis. Preparative gradient polyacrylamide gel electrophoresis can also be used to obtain pure oligosaccharides for structural studies. Future prospects for the complete se-quence analysis of oligosaccharides and polysaccharides by electrophoresis are discussed.
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofTrends in Glycoscience and Glycotechnology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleMapping and Sequencing of Oligosaccharides by Electrophoresis
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)

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