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電気泳動を使用するオリゴ糖のマッピングとシークエンス分析

dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorLiu, J.
dc.contributor.authorHan, X.-J.
dc.date1993
dc.date.accessioned2022-06-27T17:14:38Z
dc.date.available2022-06-27T17:14:38Z
dc.date.issued1993-02-01
dc.identifier.citationMapping and Sequencing of Oligosaccharides by Electrophoresis R.J. Linhardt, J. Liu, X. -J. Han, Trends in Glycoscience and Glycotechnology, 5, 181-192 (1993).
dc.identifier.issn9157352
dc.identifier.urihttps://doi.org/10.4052/tigg.5.181
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5978
dc.descriptionTrends in Glycoscience and Glycotechnology, 5, 181-192
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractThe analysis of oligosaccharides and polysaccharides takes on increased importance with the discovery of each new biological activity attributable to this important class of biopolymers. Our understanding of other biopolymers, pro-teins and nucleic acids, has relied heavily on electrophoresis. This minireview describes the application of capillary electro-phoresis and gradient polyacrylamide gel electrophoresis to saccharide compositional analysis, oligosaccharide mapping and oligosaccharide and polysaccharide sequencing. These ap-proaches are applicable to both acidic polysaccharides (i.e., glycosaminoglycans) and neutral, glycoprotein-derived oligo-saccharides. Separation by electrophoresis can be driven by the inherent charge of a carbohydrate, through the complexation of a carbohydrate with a charged molecule, or through derivati-zation reactions to prepare charged carbohydrate conjugates. New approaches including fluorescence labeling provide the high detection sensitivity required for microanalysis. The high resolution necessary for oligosaccharide analysis can be achieved using capillary electrophoresis or two-dimensional gel electrophoresis. Preparative gradient polyacrylamide gel electrophoresis can also be used to obtain pure oligosaccharides for structural studies. Future prospects for the complete se-quence analysis of oligosaccharides and polysaccharides by electrophoresis are discussed.
dc.description.abstractオリコ糖や多糖の分析は、オリコ糖や多糖に起因する生物活性の発見とともにますますその重要性が認識されつつある。タンパク質や核酸など、その他の生体高分子は電気泳動を利用する分析法に大きく依存レている。本論文ではキャピラリー電気泳動およびグラジエントポリアクリルアミドゲル電気泳動を用いる構成糖分析、オリゴ糖マッピング、オリコ糖および多糖のシークエンス分析について紹介する。今回紹介する方法は酸性多糖(グリコサミノグリカン)や中性の糖タンパク質に由来するオリコ聴に適用できる。糖質を電気泳動により分離する手段として、糖が本来持っている電荷、糖とキャリアー緩衝液中の荷電分子との錯体形成、および誘導体化により糖質に電荷を導入するという3種類の方法を利用することができる。蛍光標識などの最近開発された方法は、ミクロ分析が必要とされる高感度分析を可能にする。オリコ糖の分析に必要とされる高い分離能はキャピラリー電気泳動や二次元ゲル電気泳動により達成される。分取グラジエントゲル電気泳動は構造解析用のオリコ糖を純粋に得るために有用な手段となる。電気泳動を用いるオリゴ糖や多糖の完全シークエンス分析の将来についても述べる。
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofTrends in Glycoscience and Glycotechnology
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleMapping and Sequencing of Oligosaccharides by Electrophoresis
dc.title電気泳動を使用するオリゴ糖のマッピングとシークエンス分析
dc.typeArticle
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.4052/tigg.5.181
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages181-192
rpi.description.volume5


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