Structure Elucidation of a Novel Acidic Tetrasaccharide and Hexasaccharide Derived From a Chemically Modified Heparin
Authors
Desai, Umesh R.
Wang, Hui Ming
Kelly, Therese R.
Linhardt, Robert J.
ORCID
https://orcid.org/0000-0003-2219-5833
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Other Contributors
Issue Date
1993-03-17
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Degree
Terms of Use
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Full Citation
Nature of the Interaction of Heparin with Acidic Fibroblast Growth Factor, H. Mach, D.B. Volkin, C.J. Burke, C.R. Middaugh, R.J. Linhardt, J. Fromm, D. Loganathan, L. Mattsson, Biochemistry, 32, 5480-5489 (1993).
Abstract
In the course of development of potentially more bioactive forms of heparin for use as an anticoagulant, we had occasion to prepare a modified heparin first reported by Jaseja et al.‘. Treatment of unfractionated bovine lung heparin with an alkaline solution at high temperature (105-110°C) for 24 h, according to method developed by Rej and Perlin3, results in a modified heparin. To structurally characterize the sample of modified heparin, it was depolymerized with heparinase (heparin lyase I, EC 4.2.2.7). This resulted in the isolation of the hitherto unreported tetrasaccharide and hexasaccharide. This note describes the isolation and structure elucidation of two novel oligosaccharides. The solution conformation of these oligosaccharides may aid in developing a better understanding of the conformational preferences of the uranic acid residues.
Description
Carbohydrate Research, 241, 249-259
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Carbohydrate Research
https://harc.rpi.edu/
Rensselaer Polytechnic Institute, Troy, NY
Carbohydrate Research
https://harc.rpi.edu/
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