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Capillary Zone Electrophoresis for the Quantitation of Oligosaccharides Formed Through the Action of Chitinase

dc.contributor.authorLee, K.B.
dc.contributor.authorKim, Y.S.
dc.contributor.authorLinhardt, Robert J.
dc.date1991
dc.date.accessioned2022-06-27T17:14:58Z
dc.date.available2022-06-27T17:14:58Z
dc.date.issued1991-01-01
dc.identifier.citationCapillary Zone Electrophoresis for the Quantitation of Oligosaccharides Formed Through the Action of Chitinase, K.B. Lee, Y.S. Kim, R.J. Linhardt, Electrophoresis, 12, 636-640 (1991).
dc.identifier.issn15222683
dc.identifier.issn1730835
dc.identifier.urihttps://doi.org/10.1002/elps.1150120907
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5996
dc.descriptionElectrophoresis, 12, 636-640
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractCapillary zone electrophoresis with fluorescence detection was used to analyze the products formed by chitinase acting on N-acetylchitooligosaccharide-fluorescent conjugates. Six oligosaccharides of the structure [N-acetylglucosamine(1----4)]n (where n = 1-6) were conjugated to 7-amino-1,3-naphthalene disulfonic acid by reductive amination. Each oligosaccharide-fluorescent conjugate was purified by preparative gradient polyacrylamide gel electrophoresis, semi-dry electrotransfer to a positively-charged nylon membrane and recovered by washing the membrane with salt solution. The products formed by treating each oligosaccharide-fluorescent conjugate with chitinase were analyzed by capillary zone electrophoresis. The chitinase treatment hexasaccharide-fluorescent conjugate was also examined kinetically to study the action pattern of this enzyme.
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1002/elps.1150120907
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofELECTROPHORESIS
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleCapillary Zone Electrophoresis for the Quantitation of Oligosaccharides Formed Through the Action of Chitinase
dc.typeArticle
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dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1002/elps.1150120907
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages636-640
rpi.description.volume12


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